Abe Chisato, Ikeda Saiko, Uchida Tomono, Yamashita Kanae, Ichikawa Tomio
Department of Nutritional Sciences, Nagoya University of Arts and Sciences, Nissin 470-0196, Japan.
J Nutr. 2007 Feb;137(2):345-50. doi: 10.1093/jn/137.2.345.
The aim of this experiment was to clarify the contribution of the alpha-tocopherol transfer activity of lipoprotein lipase (LPL) to vitamin E transport to tissues in vivo. We studied the effect of Triton WR1339, which prevents the catabolism of triacylglycerol-rich lipoproteins by LPL on vitamin E distribution in rats. Vitamin E-deficient rats fed a vitamin E-free diet for 4 wk were injected with Triton WR1339 and administered by oral gavage an emulsion containing 10 mg of alpha-tocopherol, 10 mg of gamma-tocopherol, or 29.5 mg of a tocotrienol mixture with 200 mg of sodium taurocholate, 200 mg of triolein, and 50 mg of albumin. alpha-Tocopherol was detected in the serum and other tissues of the vitamin E-deficient rats, but gamma-tocopherol, alpha- and gamma-tocotrienol were not detected. Triton WR1339 injection elevated (P<0.05) the serum alpha-tocopherol concentration and inhibited (P<0.05) the elevation of alpha-tocopherol concentration in the liver, adrenal gland, and spleen due to the oral administration of alpha-tocopherol. Neither alpha-tocopherol administration nor Triton WR1339 injection affected (P>or=0.05) the alpha-tocopherol concentration in the perirenal adipose tissue, epididymal fat, and soleus muscle despite a high expression of LPL in the adipose tissue and muscle. These data show that alpha-tocopherol transfer activity of LPL in adipose tissue and muscle is not important for alpha-tocopherol transport to the tissue after alpha-tocopherol intake or that the amount transferred is small relative to the tissue concentration. Furthermore, Triton WR1339 injection tended to elevate the serum gamma-tocopherol (P=0.071) and alpha-tocotrienol (P=0.053) concentrations and lowered them (P<0.05) in the liver and adrenal gland of rats administered gamma-tocopherol or alpha-tocotrienol. These data suggest that lipolysis of triacylglycerol-rich chylomicron by LPL is necessary for postprandial vitamin E transport to the liver and subsequent transport to the other tissues.
本实验的目的是阐明脂蛋白脂肪酶（LPL）的α-生育酚转运活性对体内维生素E向组织转运的贡献。我们研究了Triton WR1339对大鼠维生素E分布的影响，Triton WR1339可阻止富含三酰甘油的脂蛋白被LPL分解代谢。给缺乏维生素E的大鼠喂食不含维生素E的饲料4周后，注射Triton WR1339，并通过口服灌胃给予含有10 mgα-生育酚、10 mgγ-生育酚或29.5 mg生育三烯酚混合物以及200 mg牛磺胆酸钠、200 mg三油酸甘油酯和50 mg白蛋白的乳剂。在缺乏维生素E的大鼠血清和其他组织中检测到了α-生育酚，但未检测到γ-生育酚、α-和γ-生育三烯酚。注射Triton WR1339使血清α-生育酚浓度升高（P<0.05），并抑制了因口服α-生育酚导致的肝脏、肾上腺和脾脏中α-生育酚浓度的升高（P<0.05）。尽管脂肪组织和肌肉中LPL表达较高，但给予α-生育酚或注射Triton WR1339均未影响（P≥0.05）肾周脂肪组织、附睾脂肪和比目鱼肌中的α-生育酚浓度。这些数据表明，脂肪组织和肌肉中LPL的α-生育酚转运活性对于摄入α-生育酚后其向组织的转运并不重要，或者相对于组织浓度而言转运量较小。此外，注射Triton WR1339使给予γ-生育酚或α-生育三烯酚的大鼠血清γ-生育酚（P=0.071）和α-生育三烯酚（P=0.053）浓度有升高趋势，而在肝脏和肾上腺中则使其降低（P<0.05）。这些数据表明，LPL对富含三酰甘油的乳糜微粒进行脂解作用对于餐后维生素E向肝脏的转运以及随后向其他组织的转运是必要的。
