Brinkman Joeli A, Xu Xuemei, Kast W Martin
Department of Molecular Microbiology & Immunology, Norris Comprehensive Cancer Center, University of Southern California, 1501 San Pablo Street, ZNI 245, Los Angeles, CA 90033, USA.
Vaccine. 2007 Apr 30;25(17):3437-44. doi: 10.1016/j.vaccine.2006.12.045. Epub 2007 Jan 10.
A majority of cervical cancers are associated with Human Papillomavirus (HPV)-16. A DNA vaccine (E7IR) was designed for prophylactic and therapeutic treatment of HPV-16+ tumors containing two repeats of the E7 gene to inactivate transformation and duplicate available epitopes. Mice were vaccinated then tumor challenged, or challenged and then immunized and monitored for tumor volume and survival. Splenocytes were utilized for in vivo CTL assays. The E7IR vaccine demonstrated decreased tumor volume and enhanced survival in prophylactic and therapeutic experiments and improved CTL-mediated lysis. The E7IR vaccine shows promise in prevention of tumor formation and elimination of established tumors.
大多数宫颈癌与人类乳头瘤病毒(HPV)-16相关。设计了一种DNA疫苗(E7IR)用于预防和治疗含有两个E7基因重复序列的HPV-16+肿瘤,以灭活转化并复制可用的表位。给小鼠接种疫苗后进行肿瘤攻击,或者先进行肿瘤攻击,然后免疫,并监测肿瘤体积和存活情况。脾细胞用于体内细胞毒性T淋巴细胞(CTL)检测。E7IR疫苗在预防和治疗实验中显示出肿瘤体积减小和存活率提高,并改善了CTL介导的裂解作用。E7IR疫苗在预防肿瘤形成和消除已形成的肿瘤方面显示出前景。