Arnold W J, Soule H D, Russo J
In Vitro. 1976 Jan;12(1):57-64. doi: 10.1007/BF02832794.
A fine structural study was made of cells from the epithelioid MCF-8/5-2A cell line derived from a MuMTV-free D2 transplantable hyperplastic outgrowth. Electron microscopy shows the cells to be truly epithelial with many cell-to-cell junctions and microvilli. The cells are similar in many respects to normal mouse mammary gland and some of the conventional mammary tumor derived cell lines. This study supports previous observations of the absence of MuMTV in MCF-8 within the limits of morphological detection, and demonstrates the presence of numerous virus particles within, or budding into, cisternae of the endoplasmic reticulum and nuclear envelope. These intracisternal A particles have not been previously described in such abundance in mammary tumor tissue culture cells.
对源自无MuMTV的D2可移植增生性赘生物的类上皮MCF-8/5-2A细胞系的细胞进行了精细结构研究。电子显微镜显示这些细胞是真正的上皮细胞，具有许多细胞间连接和微绒毛。这些细胞在许多方面与正常小鼠乳腺以及一些传统的乳腺肿瘤衍生细胞系相似。本研究在形态学检测范围内支持了之前关于MCF-8中不存在MuMTV的观察结果，并证明在内质网和核膜的池内或向其出芽的过程中存在大量病毒颗粒。这些池内A颗粒此前尚未在乳腺肿瘤组织培养细胞中如此大量地被描述过。
