在 5-溴脱氧尿苷取代染色体中的姐妹染色单体交换的性质。

On the nature of sister-chromatid exchanges in 5-bromodeoxyuridine-substituted chromosomes.

机构信息

Instituto de Biología Celular (CSIC) Velázquez 144, Madrid-6, Spain.

出版信息

Genetics. 1979 Aug;92(4):1251-64. doi: 10.1093/genetics/92.4.1251.

DOI:10.1093/genetics/92.4.1251

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC1214069/

Abstract

Sister-chromatid exchanges (SCE) were studied in allium cepa L. meristematic cells at the second and third divisions after BrdUrd-substitution during just the first or during the second and third cycles, respectively. The observed SCE nonreciprocal/reciprocal ratios detected at the third division in both experiments, as well as comparison of the lowest SCE frequency observed per cycle and expressed per picogram of DNA with data from different species expressed accordingly, strongly suggest that most of the exchanges detected in BrdUrd-substituted chromosomes are BrdUrd-dependent events. Hypotheses suggesting some different mechanisms are discussed to explain the formation of these BrdUrd-dependent SCEs.

摘要

姐妹染色单体交换（SCE）在 BrdUrd 替代后的第一次或第二次和第三次分裂中分别在洋葱根尖分生细胞中进行研究。在这两个实验中，在第三次分裂中观察到的 SCE 非相互/相互比值，以及每个周期观察到的最低 SCE 频率与每个皮克 DNA 的比值与相应表达的不同物种的数据进行比较，强烈表明在 BrdUrd 替代染色体中检测到的大多数交换是 BrdUrd 依赖性事件。讨论了一些不同的假说来解释这些 BrdUrd 依赖性 SCE 的形成。

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