视神经再生过程中金鱼视网膜中转谷氨酰胺酶的上调。

Upregulation of transglutaminase in the goldfish retina during optic nerve regeneration.

作者信息

Sugitani Kayo, Matsukawa Toru, Maeda Ari, Kato Satoru

机构信息

Department of Molecular Neurobiology, Graduate School of Medicine, Kanazawa University, 13-1 Takara-machi, Kanazawa 920-8640, Japan.

出版信息

Adv Exp Med Biol. 2006;572:525-30. doi: 10.1007/0-387-32442-9_73.

DOI:10.1007/0-387-32442-9_73

PMID:17249619

Abstract

To elucidate the molecular involvement of transglutaminase (TG) in central nervous system (CNS) regeneration, we cloned a full-length cDNA for neural TG (TG(N)) from axotomized goldfish retinas and produced a recombinant TG(N) protein from this cDNA. The levels of TG(N) mRNA and protein were increased at 10-30 days after optic nerve transection, and this increase in TG(N) was only localized in the ganglion cells in goldfish retinas. In retinal explant cultures, the recombinant TG(N) protein induced a drastic enhancement of neurite outgrowth, while TG(N)-specific RNAi significantly suppressed this neurite outgrowth. Taken together, these data strongly indicate that TG(N) is a key regulatory molecule for CNS regeneration.

摘要

为阐明转谷氨酰胺酶（TG）在中枢神经系统（CNS）再生中的分子作用，我们从视神经切断的金鱼视网膜中克隆了神经TG（TG(N)）的全长cDNA，并从该cDNA制备了重组TG(N)蛋白。视神经横断后10 - 30天，TG(N) mRNA和蛋白水平升高，且这种TG(N)的增加仅局限于金鱼视网膜的神经节细胞中。在视网膜外植体培养中，重组TG(N)蛋白显著促进了神经突生长，而TG(N)特异性RNA干扰则显著抑制了这种神经突生长。综上所述，这些数据有力地表明TG(N)是CNS再生的关键调节分子。