Cai WeiSong, Zhao HongYu, Guo JunBin, Li Yong, Yuan ZhengWei, Wang WeiLin
Congenital Malformation Lab, Pediatric Surgery, The Second Affiliated Hospital (Shengjing Hospital), China Medical University, Shenyang, 110004, People's Republic of China,
Childs Nerv Syst. 2007 May;23(5):549-54. doi: 10.1007/s00381-006-0289-y. Epub 2007 Jan 25.
To observe the morphological features of the lumbosacral neural tube defects (NTDs) induced by all-trans retinoic acid (atRA) and to explore the pathogenesis of these defects.
Rat embryos with lumbosacral NTDs were obtained by treating pregnant rats with administration of atRA. Rat embryos were obtained by cesarean. Fetuses were sectioned and stained with hematoxylin-eosin (H&E). Relevant structures including caudal neural tube were examined. In the atRA-treated rats, about 48% embryos showed lumbosacral NTDs. There appeared a dorsally and rostrally situated, neural-plate-like structure (myeloschisis) and a ventrally and caudally located cell mass containing multiple canals (hamartoma) in the lumbosacral NTDs induced by atRA.
Retinoic acid could disturb the notochord and tail bud development in the process of primary and secondary neurulation in rat embryos, which cause lumbosacral NTDs including myeloschisis and hamartoma. The morphology is very similar to that happens in humans.
观察全反式维甲酸(atRA)诱导的腰骶部神经管缺陷(NTDs)的形态学特征,并探讨这些缺陷的发病机制。
通过给孕鼠腹腔注射atRA来获得具有腰骶部NTDs的大鼠胚胎。通过剖宫产获取大鼠胚胎。将胎儿进行切片并用苏木精-伊红(H&E)染色。检查包括尾神经管在内的相关结构。在经atRA处理的大鼠中,约48%的胚胎出现腰骶部NTDs。在由atRA诱导的腰骶部NTDs中,出现了一个位于背侧和头侧的神经板样结构(脊髓裂)以及一个位于腹侧和尾侧的包含多个管腔的细胞团(错构瘤)。
维甲酸可在大鼠胚胎原肠胚形成和神经管形成过程中干扰脊索和尾芽的发育,从而导致包括脊髓裂和错构瘤在内的腰骶部NTDs。其形态与人类发生的情况非常相似。