两种针对日本血吸虫成虫和童虫皮层的单克隆抗体的特性分析

Characterization of two monoclonal antibodies against teguments of adult and schistosomula of Schistosoma japonicum.

作者信息

Viyanant V, Sobhon P, Upatham E S, Kusamran T, Kittigul C, Panuwatsuk W, Ardseungnoen P, Anatawara S

机构信息

Department of Biology, Faculty of Science, Mahidol University, Bangkok, Thailand.

出版信息

Asian Pac J Allergy Immunol. 1991 Dec;9(2):101-6.

PMID:1725481

Abstract

Two monoclonal antibodies (McAbs) were produced from BALB/c mice hyperimmunized with tegumental extract of Schistosoma japonicum (Chinese strain). The two McAbs were characterized with regard to antibody isotype, antigen binding specificity and parasite stage specificity. One McAb, 8G9-5, was identified as IgM, whereas the other McAb, 9E7, was determined to be IgG2a. Immunoblotting assay indicated that McAb 8G9-5 binds strongly to the band of tegumental antigens of Mr 64 kDa but also binds weakly to other bands at 116, 105, 97, 54, 50, 47 and 45 kDa, whereas 9E7 McAb reacts specifically at Mr 54 kDa. Anatomical localization of the antigens in the adult worm by indirect immunofluorescence assay indicated that the target epitopes of McAb 8G9-5 are in the intra-tegumental structure, whereas the McAb 9E7 epitope is on the surface membrane. The two McAbs also react at similar sites within the teguments of schistosomula and lung worms.

摘要

用日本血吸虫（中国株）皮层提取物对BALB/c小鼠进行超免疫，制备了两种单克隆抗体（McAbs）。对这两种单克隆抗体的抗体同种型、抗原结合特异性和寄生虫阶段特异性进行了表征。一种单克隆抗体8G9-5被鉴定为IgM，而另一种单克隆抗体9E7被确定为IgG2a。免疫印迹分析表明，单克隆抗体8G9-5与64 kDa的皮层抗原条带有强烈结合，但也与116、105、97、54、50、47和45 kDa的其他条带有弱结合，而9E7单克隆抗体在54 kDa处有特异性反应。通过间接免疫荧光测定法对成虫中抗原的解剖定位表明，单克隆抗体8G9-5的靶表位位于皮层内结构，而单克隆抗体9E7的表位位于表面膜上。这两种单克隆抗体在童虫和肺期虫体的皮层内相似部位也有反应。