胶原酶在正常大鼠组织中的分布。

The distribution of collagenase in normal rat tissues.

作者信息

Montfort I, Pérez-Tamayo R

出版信息

J Histochem Cytochem. 1975 Dec;23(12):910-20. doi: 10.1177/23.12.172556.

DOI:10.1177/23.12.172556

Abstract

A rabbit monospecific anti-rat uterus collagenase antibody has been used to study the distribution of collagenase in normal rat tissues by immunohistochemical methods. Indirect staining was performed with fluorescein-conjugated goat anti-rabbit immunoglobulin G antibody. The organs studied were brain, lung, myocardium, liver, spleen, kidney, adrenal, testes, uterus, xiphoid cartilage, tail tendon, skeletal (triceps) muscle and skin. Collagenase is widely present throughout the connective tissue structures in all organs examined. The enzyme is apparently bound to collagen fibers, reticulum fibers and basement membranes. The results suggest that control of collagenase activity depends on factors other than the presence of the enzyme in tissues.

摘要

一种兔抗大鼠子宫胶原酶单特异性抗体已被用于通过免疫组织化学方法研究胶原酶在正常大鼠组织中的分布。用荧光素偶联的山羊抗兔免疫球蛋白G抗体进行间接染色。所研究的器官有脑、肺、心肌、肝、脾、肾、肾上腺、睾丸、子宫、剑突软骨、尾腱、骨骼肌（肱三头肌）和皮肤。在所有检查的器官中，胶原酶广泛存在于整个结缔组织结构中。该酶显然与胶原纤维、网状纤维和基底膜结合。结果表明，胶原酶活性的控制取决于组织中酶的存在以外的因素。