氯沙坦对糖尿病大鼠肾脏中血管紧张素II及其1型受体水平的影响

[Effects of losartan on the levels of angiotensin II and its type-1 receptor in diabetic rat kidney].

作者信息

Wu Wen, Ding He-lin, Chen Li-hong, Fu Zu-zhi

机构信息

Department of Endocrinology, Institute of Geriatrics, People's Hospital of Guangdong Province, Guangzhou 510080, China.

出版信息

Nan Fang Yi Ke Da Xue Xue Bao. 2006 Dec;26(12):1742-4.

PMID:17259110

Abstract

OBJECTIVE

To investigate the effect of losartan, an angiotensin II type-1 receptor (AT1R) antagonist, on the levels of angiotensin II (Ang II) and AT1R in diabetic rat kidney.

METHODS

Male Wistar rats were divided into 3 groups, group A (n=11) served as the control group, group B (n=11) included the diabetic rats (induced by intraperitoneal injection of streptozotocin) without any therapy, and group C (n=9) diabetic rats treated with losartan. After 18 weeks of treatment, the kidneys were taken from all the rats to measure the expression of AT1R mRNA by RT-PCR and detect the Ang II level. Blood was also drawn from the heart to measure Ang II level, and 24-hour urine was collected to measure albumin level (urine albumin excretion, UAE) with rat albumin enzyme immunoassay kit.

RESULTS

The blood and renal Ang II levels showed no significant difference between the 3 groups. The expression of renal AT1R mRNA in group B (0.62-/+0.17) was significantly lower than that in group A (1.13-/+0.82, P<0.01) and group C (1.13-/+0.62,P<0.01). UAE in group B (2.18-/+1.98 mg) was significantly higher than that in group A (0.41-/+0.47 mg/d, P<0.01) and C (0.65 -/+0.89 mg/d, P<0.01).

CONCLUSION

Losartan can increase the expression of AT1R mRNA in diabetic rat kidneys without altering the blood and renal Ang II levels.

摘要

目的

研究血管紧张素II 1型受体（AT1R）拮抗剂氯沙坦对糖尿病大鼠肾脏中血管紧张素II（Ang II）水平及AT1R的影响。

方法

将雄性Wistar大鼠分为3组，A组（n = 11）为对照组，B组（n = 11）为未接受任何治疗的糖尿病大鼠（通过腹腔注射链脲佐菌素诱导），C组（n = 9）为接受氯沙坦治疗的糖尿病大鼠。治疗18周后，取所有大鼠的肾脏，通过逆转录聚合酶链反应（RT-PCR）检测AT1R mRNA的表达，并检测Ang II水平。同时从心脏采血检测Ang II水平，并收集24小时尿液，用大鼠白蛋白酶免疫分析试剂盒检测白蛋白水平（尿白蛋白排泄量，UAE）。

结果

3组之间血液和肾脏的Ang II水平无显著差异。B组肾脏AT1R mRNA的表达（0.62±0.17）显著低于A组（1.13±0.82，P<0.01）和C组（1.13±0.62，P<0.01）。B组的UAE（2.18±1.98mg）显著高于A组（0.41±0.47mg/d，P<0.01）和C组（0.65±0.89mg/d，P<0.01）。