活动性克罗恩病患者血液中CD16(+)和CD56(+)单核细胞亚群增加。

Increased subpopulations of CD16(+) and CD56(+) blood monocytes in patients with active Crohn's disease.

作者信息

Grip Olof, Bredberg Anders, Lindgren Stefan, Henriksson Gunnel

机构信息

Division of Gastroenterology and Hepatology, Department of Clinical Sciences Malmö, Lund University, University Hospital MAS, Malmö, Sweden.

出版信息

Inflamm Bowel Dis. 2007 May;13(5):566-72. doi: 10.1002/ibd.20025.

DOI:10.1002/ibd.20025

PMID:17260384

Abstract

BACKGROUND

Circulating monocytes may be subdivided according to the presence or absence of the Fcgamma receptor CD16 and the neural cell adhesion molecule CD56. Monocytes classified into these subpopulations are characterized by distinct phenotypic and functional features. We hypothesized that patients with active Crohn's disease differ in their peripheral monocyte subpopulations.

METHODS

Using flow cytometry we investigated the expression of CD16 and CD56 on circulating monocytes in 11 patients with active Crohn's disease and 11 controls. These monocyte subpopulations were then analyzed for expression of the chemokine receptor fractalkine, CX(3)CR1, and the monocyte chemoattractant protein-1, CCR2.

RESULTS

We found a median 3.7-fold increase in the number of CD16(+) monocytes related to the population with high expression of the pattern recognition receptor CD14 compared to that in the controls (P < 0.001). By studying the percentage of monocytes expressing CX(3)CR1, and their relative fluorescence intensity (RFI), we found significant differences, with both the highest percentage and the highest RFI in the CD14(low)CD16(+) subpopulation, whereas the CD14(high)CD16(+) subgroup represented an intermediate population. Inversely, CCR2 expression was highest in the populations with high expression of CD14, whereas the CD14(low)CD16(+) subpopulation showed the lowest percentage and the lowest RFI for CCR2. We found the percentage of CD14(+)CD56(+) monocytes in patients with active Crohn's disease to be increased 2.7 times compared to the controls (P = 0.011).

CONCLUSIONS

These results show that subsets of peripheral monocytes with a more mature phenotype are expanded in patients with active Crohn's disease.

摘要

背景

循环单核细胞可根据是否存在Fcγ受体CD16和神经细胞黏附分子CD56进行细分。分类到这些亚群中的单核细胞具有独特的表型和功能特征。我们推测，活动期克罗恩病患者的外周单核细胞亚群存在差异。

方法

我们采用流式细胞术研究了11例活动期克罗恩病患者和11例对照者循环单核细胞上CD16和CD56的表达情况。然后分析这些单核细胞亚群中趋化因子受体fractalkine、CX(3)CR1和单核细胞趋化蛋白-1、CCR2的表达情况。

结果

我们发现，与对照组相比，模式识别受体CD14高表达人群中CD16(+)单核细胞数量的中位数增加了3.7倍（P < 0.001）。通过研究表达CX(3)CR1的单核细胞百分比及其相对荧光强度（RFI），我们发现存在显著差异，CD14(低)CD16(+)亚群的百分比和RFI均最高，而CD14(高)CD16(+)亚组代表中间人群。相反，CCR2表达在CD14高表达人群中最高，而CD14(低)CD16(+)亚群的CCR2百分比和RFI最低。我们发现，活动期克罗恩病患者中CD14(+)CD56(+)单核细胞的百分比相比对照组增加了2.7倍（P = 0.011）。