长春花细胞异养培养中长春多灵存在的质谱证据。

Mass Spectral Evidence of the Occurrence of Vindoline in Heterotrophic Cultures of Catharanthus roseus Cells.

作者信息

Naaranlahti T, Lapinjoki S P, Huhtikangas A, Toivonen L, Kurtén U, Kauppinen V, Lounasmaa M

机构信息

Department of Pharmaceutical Chemistry, University of Kuopio, FOB 6, SF-70211 Kuopio, Finland.

出版信息

Planta Med. 1989 Apr;55(2):155-7. doi: 10.1055/s-2006-961911.

DOI:10.1055/s-2006-961911

PMID:17262331

Abstract

Vindoline concentrations in the leaves of 70 CATHARANTHUS ROSEUS of 3 cultivars were analyzed by HPLC, and 3 plants were selected for starting callus cultures on different media. When the initial calli were analyzed using a vindoline-specific RIA, the assay suggested a vindoline content of about 10 (-5)% dry weight for one-third of the first 60 cultures examined. Due to the unexpectedly high incidence of vindoline-positive calli, the screening programme was discontinued and efforts were concentrated on verifying the existence of this alkaloid in the cells. Suspension cultures derived from the 5 most immunopositive calli in an alkaloid production medium were analyzed by HPLC and GC/MS. Comparison with reference material showed that the heterotrophic suspension cultures contained a compound identical to vindoline.

摘要

采用高效液相色谱法分析了3个品种的70株长春花叶片中的长春多灵浓度，并选取3株在不同培养基上开始愈伤组织培养。当使用长春多灵特异性放射免疫分析法对初始愈伤组织进行分析时，该分析表明，在所检测的前60个培养物中，三分之一的愈伤组织中长春多灵含量约为干重的10(-5)%。由于长春多灵阳性愈伤组织的发生率出乎意料地高，筛选程序停止，工作集中在验证细胞中这种生物碱的存在。通过高效液相色谱法和气相色谱/质谱联用仪对在生物碱生产培养基中由5个免疫阳性最强的愈伤组织衍生的悬浮培养物进行了分析。与参考物质的比较表明，异养悬浮培养物中含有一种与长春多灵相同的化合物。