Nghiêm H O, Hill J, Changeux J P
U.A. CNRS D 1284, Neurobiologie Mol:ecularie, Institut Pasteur, Paris, France.
Development. 1991 Dec;113(4):1059-67. doi: 10.1242/dev.113.4.1059.
Analysis of the relative amounts of the acetylcholine receptros (AChR) and of the 43K protein present in the membrane of developing electrocyte shows that massive accumulation of 43K protein is not required for induction of early AChR clustering. Furthermore, we demonstrate the existence o of cytosol- and membrane-associated 43K polypeptide pools in Torpedo electrocyte. Epitope analysis shows that both pools of 43K protein are related to the long mRNA transcript and share similar antigenic determinants distributed throughout the protein sequence. Their partition between the cytosol and membrane fractions abruptly increases in favor of the membrane during the postsynaptic maturation phase of development, supporting a role for 43K protein in the stabilization and maintenance of the postsynaptic domain.
对发育中的电细胞细胞膜中乙酰胆碱受体（AChR）和43K蛋白的相对含量分析表明，早期AChR聚集的诱导并不需要43K蛋白的大量积累。此外，我们证明了电鳐电细胞中存在细胞溶质和膜相关的43K多肽池。表位分析表明，43K蛋白的两个池都与长mRNA转录本相关，并且在整个蛋白质序列中共享相似的抗原决定簇。在发育的突触后成熟阶段，它们在细胞溶质和膜部分之间的分配突然增加，有利于膜，这支持了43K蛋白在突触后结构域的稳定和维持中的作用。
