前体物质对赪桐叶片-嫩梢器官培养物生长和生物碱生成的影响。

Effect of Precursors on Growth and Alkaloid Production in Chinchona ledgeriana Leaf-Shoot Organ Cultures.

机构信息

Department of Medicinal Chemistry and Pharmacognosy, University of Minnesota, Minneapolis, Minnesota 55455, U.S.A.

出版信息

Planta Med. 1988 Jun;54(3):206-10. doi: 10.1055/s-2006-962405.

DOI:10.1055/s-2006-962405

Abstract

The alkaloid contents of the serially propagated CINCHONA LEDGERIANA leaf-shoot organ cultures that were fed with various precursors, were compared to the control cultures that were grown in Murashige and Skoog's medium supplemented with 5 mg/l benzyladenine. the average total alkaloid content of the 8-week-old leaf-shoot cultures increased approximately 66% with tryptopnan; 42% with secologanin; 5% with strictosidine-type, and decreased 10% with methoxystrictosidine-type alkaloid intermediates. The radioactive precursors: L-[3'- (14)C]-tryptophan was incorporated into cinchonidine (0.04%), cinchonine (0.01%), quinidine (0.01%), and quinine (0.08%); [5- (14)C]-10-strictosidine-HCl was incorporated into cinchonidine (0.01%), cinchonine (0.003%), quinidine (0.003%), and quinine (0.005%); and [5- (14)C]-10-methoxystrictosidine.HCl was incorporated equally (0.01%) into quinidine and quinine.

摘要

经各种前体喂养的连续繁殖的金鸡纳 ledgeriana 叶芽器官培养物的生物碱含量与在补充有 5mg/l 苄基腺嘌呤的 murashige 和 skoog 培养基中生长的对照培养物进行了比较。8 周龄叶芽培养物的总生物碱含量平均增加了约 66%，色氨酸增加了 42%，secologanin 增加了 5%，而严格托品烷型生物碱中间产物减少了 10%。放射性前体：l-[3'-(14)c]-色氨酸掺入到辛可宁（0.04%）、辛可宁（0.01%）、奎宁（0.01%）和奎宁（0.08%）中；[5-（14）c]-10-严格托品烷盐酸盐掺入到辛可宁（0.01%）、辛可宁（0.003%）、奎宁（0.003%）和奎宁（0.005%）中；而[5-（14）c]-10-甲氧基严格托品烷盐酸盐则同样（0.01%）掺入到奎宁和奎宁中。