Suppr超能文献

谷胱甘肽结合反应在1-溴丙烷诱导雌性BALB/c小鼠肝毒性和免疫毒性中的作用

Role of glutathione conjugation in the hepatotoxicity and immunotoxicity induced by 1-bromopropane in female BALB/c mice.

作者信息

Lee Sang Kyu, Jeon Tae Won, Kim Yong Beom, Lee Eung Seok, Jeong Hye Gwang, Jeong Tae Cheon

机构信息

College of Pharmacy, Yeungnam University, Gyeongsan, 712-749, South Korea.

出版信息

J Appl Toxicol. 2007 Jul-Aug;27(4):358-67. doi: 10.1002/jat.1214.

Abstract

1-Bromopropane (1-BP) is used as a cleaning agent or adhesive solvent in the workplace. In the present study, the hepatotoxic and immunotoxic effects of 1-bromopropane and its conjugation with glutathione (GSH) were investigated in female BALB/c mice. The animals were treated orally with 200, 500 and 1000 mg kg(-1) of 1-BP in corn oil for a dose response study or treated orally with 1000 mg kg(-1) of 1-BP for 6, 12, 24 and 48 h for a time course study. The hepatic and splenic contents of GSH were significantly decreased by 1-BP in a dose-dependent manner. S-propyl GSH was identified in livers following treatment with 1-BP by liquid chromatography-electrospray ionization tandem mass spectrometry. When the production of conjugates from 1-BP was investigated in livers following oral treatment with 1000 mg kg(-1) of 1-BP for 6, 12, 24 and 48 h, the GSH conjugates were detected maximally 6 h after treatment. Treatment of mice with 1-BP increased the serum activity of alanine aminotransferase dose-dependently. The oral 1-BP treatment significantly suppressed the antibody response to a T-dependent antigen and the production of splenic intracellular IL-2 in response to Con A in a dose-dependent manner. The present results suggested that 1-BP could cause hepatotoxicity and immunotoxicity as well as depletion of GSH content due to the formation of GSH conjugates.

摘要

1-溴丙烷(1-BP)在工作场所用作清洁剂或粘合剂溶剂。在本研究中,对雌性BALB/c小鼠研究了1-溴丙烷及其与谷胱甘肽(GSH)结合物的肝毒性和免疫毒性作用。为进行剂量反应研究,给动物口服200、500和1000 mg/kg的1-BP玉米油溶液;为进行时间进程研究,给动物口服1000 mg/kg的1-BP玉米油溶液,持续6、12、24和48小时。1-BP以剂量依赖方式显著降低肝脏和脾脏中的GSH含量。通过液相色谱-电喷雾电离串联质谱法在1-BP处理后的肝脏中鉴定出S-丙基GSH。当口服1000 mg/kg的1-BP处理6、12、24和48小时后,在肝脏中研究1-BP结合物的生成情况时,在处理后6小时最大程度地检测到GSH结合物。用1-BP处理小鼠会使血清丙氨酸转氨酶活性呈剂量依赖性增加。口服1-BP处理以剂量依赖方式显著抑制对T依赖性抗原的抗体反应以及脾脏细胞内IL-2对刀豆蛋白A的产生。目前的结果表明,1-BP可导致肝毒性和免疫毒性以及由于GSH结合物的形成导致GSH含量的消耗。

文献AI研究员

20分钟写一篇综述,助力文献阅读效率提升50倍。

立即体验

用中文搜PubMed

大模型驱动的PubMed中文搜索引擎

马上搜索

文档翻译

学术文献翻译模型,支持多种主流文档格式。

立即体验