Yang Fang, Xiao Zhi-qiang, Zhang Xiu-zhi, Li Cui, Zhang Peng-fei, Li Mao-yu, Chen Ying, Zhu Ge-qin, Sun Yi, Liu Ying-fu, Chen Zhu-Chu
Key Laboratory of Cancer Proteomics of Chinese Ministry of Health, Xiangya Hospital, Central South University, #87 Xiangya Road, Changsha 410078, Hunan, China.
J Proteome Res. 2007 Feb;6(2):751-8. doi: 10.1021/pr0602287.
Autoantibodies against tumor antigens are promising means for cancer diagnosis and prognosis. In this study, we applied a proteomic approach to identify proteins that commonly elicit humoral response in lung squamous carcinoma (LSC). Sera from 20 newly diagnosed patients with LSC and 20 matched healthy individuals were analyzed for antibody-based reactivity against LSC proteins separated by two-dimensional electrophoresis. Autoantibodies against triosephosphate isomerase (Tim) and superoxide dismutase [Mn] (MnSOD) were detected in sera from over 20% patients with LSC but none from the normal controls. Furthermore, the occurrence of autoantibodies against Tim and MnSOD was evaluated by ELISA in an additional 40 LSC patients, 30 other types of cancer (OTC) patients, and 50 noncancer controls (NC). Results showed that frequency of autoantibody against Tim (27.5%) in LSC patients was significantly higher than that in OTC patients (6.7%, p = 0.027) and in NC (6%, p = 0.005). Likewise, frequency of autoantibody against MnSOD in LSC (20%) patients was significantly higher than that in NC (4%, p = 0.016), however, there was no significant difference when comparing to that in OTC patients (6.7%, p = 0.115). We also observed significantly increased expression and secretion of Tim and MnSOD in LSC, which possibly account for their autoantibody development. Our results indicate that autoantibody and antigen of Tim and MnSOD may be useful for screening and diagnosis of the lung squamous carcinoma.
针对肿瘤抗原的自身抗体是癌症诊断和预后的有前景的手段。在本研究中，我们应用蛋白质组学方法来鉴定在肺鳞状细胞癌（LSC）中通常引发体液免疫反应的蛋白质。分析了20例新诊断的LSC患者和20例匹配的健康个体的血清，检测其对通过二维电泳分离的LSC蛋白质的基于抗体的反应性。在超过20%的LSC患者血清中检测到针对磷酸丙糖异构酶（Tim）和超氧化物歧化酶[Mn]（MnSOD）的自身抗体，而正常对照中均未检测到。此外，通过酶联免疫吸附测定（ELISA）在另外40例LSC患者、30例其他类型癌症（OTC）患者和50例非癌症对照（NC）中评估了针对Tim和MnSOD的自身抗体的出现情况。结果显示，LSC患者中针对Tim的自身抗体频率（27.5%）显著高于OTC患者（6.7%，p = 0.027）和NC（6%，p = 0.005）。同样，LSC患者中针对MnSOD的自身抗体频率（20%）显著高于NC（4%，p = 0.016），然而，与OTC患者相比无显著差异（6.7%，p = 0.115）。我们还观察到LSC中Tim和MnSOD的表达和分泌显著增加，这可能解释了它们自身抗体的产生。我们的结果表明，Tim和MnSOD的自身抗体和抗原可能对肺鳞状细胞癌的筛查和诊断有用。
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