Arons J A, Wainwright D J, Jordon R E
Department of Surgery, University of Texas Medical School, Houston 77030.
Surgery. 1992 Jan;111(1):4-11.
Replacement of skin has long been the ultimate task for surgeons facing skin-resurfacing challenges such as thermal burns and chronic ulcerations. Autologous skin grafts have been the "gold standard" for wound closure, but in patients who are massively burned, the availability of normal skin is the limiting factor. In the past 15 years the technique of in vitro cultivation of human epidermis has been developed in an attempt to deal with the problem of extensive skin loss. Although the technique is costly and arduous, grafting patients who are severely burned with cultured epidermal autografts has proved to be a life-saving measure where few alternatives exist. Cultured allografts have promoted rapid healing and pain relief in patients with chronic ulcers. Although longer follow-up is necessary, recent evidence suggests that cultured epidermis provides a wound cover that is just as durable and esthetically acceptable as conventional split-thickness skin grafts. This article reviews the development and applications of epidermal cell culture.
对于面临诸如热烧伤和慢性溃疡等皮肤修复挑战的外科医生来说，皮肤替代长期以来一直是最终目标。自体皮肤移植一直是伤口闭合的“金标准”，但在大面积烧伤患者中，正常皮肤的可用性是限制因素。在过去15年里，人类表皮体外培养技术得到了发展，试图解决大面积皮肤缺损问题。尽管该技术成本高昂且操作艰巨，但用培养的自体表皮移植严重烧伤患者已被证明是一种在几乎没有其他选择时的救命措施。培养的异体皮促进了慢性溃疡患者的快速愈合和疼痛缓解。尽管需要更长时间的随访，但最近的证据表明，培养的表皮提供的伤口覆盖与传统的中厚皮片一样耐用且美观。本文综述了表皮细胞培养的发展及应用。
