通过Biojector无针注射进行皮内DNA免疫后小鼠对轮状病毒攻击的保护作用。
Protection of mice against rotavirus challenge following intradermal DNA immunization by Biojector needle-free injection.
作者信息
Choi Anthony H-C, Smiley Kristi, Basu Mitali, McNeal Monica M, Shao Mingyuan, Bean Judy A, Clements John D, Stout Richard R, Ward Richard L
机构信息
Division of Infectious Diseases, Cincinnati Children's Hospital Medical Center, Cincinnati, OH 45229, USA.
出版信息
Vaccine. 2007 Apr 20;25(16):3215-8. doi: 10.1016/j.vaccine.2007.01.035. Epub 2007 Jan 22.
Abstract
Mucosal administration (intranasal or oral) of a VP6 rotavirus vaccine to mice consistently elicits high levels of protection after rotavirus challenge (93->99% reductions in fecal rotavirus shedding) but only when co-administered with an effective adjuvant such as LT(R192G). Here, we showed that Biojector needle-free injection of VP6-encoded plasmids also induced protection (85-93%) when they were co-administrated with LT(R192G)-encoded plasmids. A reduction in the amount of VP6 plasmid from 50 to 10 microg reduced protection from 93 to 70%, but the immunized mice remained significantly (P<0.05) protected. Intramuscular needle injection of VP6/LT(R192G)-plasmids also induced significant protection (66%).
摘要
向小鼠黏膜给药（鼻内或口服）VP6轮状病毒疫苗，在轮状病毒攻击后始终能引发高水平的保护作用（粪便中轮状病毒排出量减少93%至99%），但只有在与有效佐剂（如LT(R192G)）共同给药时才会如此。在此，我们表明，当与LT(R192G)编码质粒共同给药时，通过Biojector无针注射VP6编码质粒也能诱导产生保护作用（85%至93%）。将VP6质粒的量从50微克减少到10微克，保护率从93%降至70%，但免疫小鼠仍受到显著保护（P<0.05）。肌肉内注射VP6/LT(R192G)质粒也能诱导显著的保护作用（66%）。
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