[优质美利奴绵羊的候选基因分析]

[Candidate gene analysis of high quality merino sheep].

作者信息

Liu Gui-Fen, Tian Ke-Chuan, Zhang En-Ping, Huang Xi-Xia, Zhang Yan-Hua

机构信息

College of Animal Science and Technology, Chinese Agricultural University, Beijing, China.

出版信息

Yi Chuan. 2007 Jan;29(1):70-4. doi: 10.1360/yc-007-0070.

DOI:10.1360/yc-007-0070

PMID:17284427

Abstract

Partial sequences of wool fiber constituent genes KAP1.1 and KAP1.3 and the exonic sequence of the KAP6.1 gene were chosen for polymerase chain reaction-single strand conformation polymorphism (PCR-SSCP) analysis to assess their ability as candidate genes during indirect selection for fine wool traits. Results show that locus W08667 in the genes (KAP1.1, KAP1.3) which code the high sulfur protein associated-protein of keratin associated-protein family is significantly correlated with fine wool quality (P < 0.05). Among the high-glycine-tyrosine keratin associated- protein, the AA and BB genotypes of W06933 are also significantly correlated with fine wool quality (P < 0.05).

摘要

选择羊毛纤维组成基因KAP1.1和KAP1.3的部分序列以及KAP6.1基因的外显子序列进行聚合酶链反应-单链构象多态性（PCR-SSCP）分析，以评估它们作为细羊毛性状间接选择候选基因的能力。结果表明，编码角蛋白相关蛋白家族高硫蛋白相关蛋白的基因（KAP1.1、KAP1.3）中的W08667位点与细羊毛品质显著相关（P<0.05）。在高甘氨酸-酪氨酸角蛋白相关蛋白中，W06933的AA和BB基因型也与细羊毛品质显著相关（P<0.05）。