Iwaki Tomoko, Morita Tomotake, Tanaka Naotaka, Giga-Hama Yuko, Takegawa Kaoru
Department of Life Sciences, Faculty of Agriculture, Kagawa University, Miki-cho, Kagawa, Japan.
Biosci Biotechnol Biochem. 2007 Feb;71(2):623-6. doi: 10.1271/bbb.60609. Epub 2007 Feb 7.
Schizosaccharomyces pombe has four alpha-amylase homologs (Aah1p-Aah4p) with a glycosylphosphatidylinositol (GPI) modification site at the C-terminal end. Disruption mutants of aah genes were tested for mislocalization of vacuolar carboxypeptidase Y (CPY), and aah3Delta was found to secrete CPY. The conversion rate from pro- to mature CPY was greatly impaired in aah3Delta, and fluorescence microscopy inidicated that a sorting receptor for CPY, Vps10p, mislocalized to the vacuolar membrane. These results indicate that aah3Delta had a defect in the retrograde transport of Vps10p, and that Aah3p is the first S. pombe specific protein required for vacuolar protein sorting.
粟酒裂殖酵母有四个α-淀粉酶同源物（Aah1p - Aah4p），在其C末端具有糖基磷脂酰肌醇（GPI）修饰位点。对aah基因的破坏突变体进行了液泡羧肽酶Y（CPY）定位错误的测试，发现aah3Δ会分泌CPY。在aah3Δ中，前体CPY向成熟CPY的转化率大大受损，荧光显微镜检查表明CPY的分选受体Vps10p定位错误到液泡膜上。这些结果表明，aah3Δ在Vps10p的逆向运输中存在缺陷，并且Aah3p是液泡蛋白分选所需的第一个粟酒裂殖酵母特异性蛋白。
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