脑出血患者的神经细胞凋亡及凋亡相关基因

[Neural apoptosis and apoptosis-related genes in intracerebral hemorrhage patients].

作者信息

Wu Cheng-han, Ding Xi-yan, Wang Hai-yan, Ye Xiao-bao, Huang Sheng-yue, Huang Ai-min, Li Hui-zhong, Wu Song-ying, Yu Ji, Yan Xiao-hua

机构信息

Department of Neurology, Second Clinical Academy of Fujian College of Traditional Chinese Medicine, Fuzhou, 350003, China.

出版信息

Zhonghua Yi Xue Za Zhi. 2006 Nov 21;86(43):3073-6.

PMID:17288840

Abstract

OBJECTIVE

To observe the neural apoptosis and expression of apoptosis-related genes in brain in order to elucidate the regulation mechanism in the perihematomal region of intracerebral hemorrhage (ICH) patients.

METHODS

Specimens of perihematomal region in human brain were obtained from 29 patients undergoing surgical evacuation of an intracerebral hematoma. Specimens of brain tissue were collected from the corpses of 6 persons within 3 hours after the accidental death. Neural apoptosis was evaluated by terminal deoxynucleotidyl transferase-mediated deoxyuridine 5' triphosphate nick end labeling (TUNEL) method and immunohistochemistry was used to detect the expression of Bcl-2, Bax, P53, and caspase-3 genes.

RESULTS

The apoptosis rates of the ICH group was 4.10 +/- 0.28, significantly higher than that of the control group (0.57 +/- 0.43, P < 0.01). The expression rate of Bcl-2 the ICH group was 2.68 +/- 0.52, significantly higher than that of the control group (1.54 +/- 0.56, P < 0.01). The expression rate of Bax of the ICH group was 3.49 +/- 0.18, significantly higher than that of the control group (0.96 +/- 0.27, P < 0.01). The expression of P53 was 4.12 +/- 0.63, significantly higher than that of the control group (0.96 +/- 0.71, P < 0.01). The expression of caspase-3 of the ICH group was 3.50 +/- 0.25, significantly higher than that of the control group (0.74 +/- 0.73, P < 0.01). The expression levels of Bcl-2 and P53 were negatively correlated with the apoptosis rate (both P < 0.01), while the expression levels of Bax and caspase-3, and the Bax/Bcl-2 ratio were positively correlated with the apoptosis rate in perihematomal region of ICH patients (all P < 0.01).

CONCLUSION

Apoptosis is involved in the delayed brain injury after ICH in human and is the main factor of delayed neural death. Some of the genes take part in the regulation of neural apoptosis: Bax and caspase-3 hasten the apoptosis while Bcl-2 and P53 restrain it.

摘要

目的

观察脑内神经细胞凋亡及凋亡相关基因的表达，以阐明脑出血（ICH）患者血肿周围区域的调控机制。

方法

选取29例行脑内血肿清除术患者的脑血肿周围区域标本。另取6例意外死亡者死后3小时内的脑组织标本。采用末端脱氧核苷酸转移酶介导的dUTP缺口末端标记法（TUNEL法）评估神经细胞凋亡情况，并用免疫组织化学法检测Bcl-2、Bax、P53和caspase-3基因的表达。

结果

ICH组凋亡率为4.10±0.28，显著高于对照组（0.57±0.43，P<0.01）。ICH组Bcl-2表达率为2.68±0.52，显著高于对照组（1.54±0.56，P<0.01）。ICH组Bax表达率为3.49±0.18，显著高于对照组（0.96±0.27，P<0.01）。P53表达为4.12±0.63，显著高于对照组（0.96±0.71，P<0.01）。ICH组caspase-3表达为3.50±0.25，显著高于对照组（0.74±0.73，P<0.01）。ICH患者血肿周围区域Bcl-2和P53表达水平与凋亡率呈负相关（均P<0.01），而Bax和caspase-3表达水平以及Bax/Bcl-2比值与凋亡率呈正相关（均P<0.01）。