Roychoudhury Sudipta, Isawa Haruhiko, Hoshino Keita, Sasaki Toshinori, Saito Noriko, Sawabe Kyoko, Kobayashi Mutsuo
Department of Medical Entomology, National Institute of Infectious Diseases, Toyama 1-23-1, Shinjuku-ku, Tokyo 162-8640, Japan.
Parasitol Int. 2007 Jun;56(2):113-8. doi: 10.1016/j.parint.2006.12.006. Epub 2007 Jan 9.
This study on the ultrastructure of the oocysts of four isolated species of Ascogregarina (A. taiwanensis (Lien and Levine) (Eugregarinidae: Lecudinidae) from Aedes albopictus (Skuse), A. culicis (Ross) (Eugregarinidae: Lecudinidae) from Aedes aegypti (L.), A. armigerei (Eugregarinidae: Lecudinidae) from Armigeres subalbatus (Coquillet), and Ascogregarina sp. (Eugregarinidae: Lecudinidae) from Ochlerotatus japonicus japonicus (Theobald)) using a scanning electron microscope revealed significant differences in size and in surface structure. The average length of the oocyst was greatest in A. armigerei (13.2+/-0.2 microm) (mean+/-SD) and least in A. culicis (8.8+/-0.4 microm). Oocysts were of moderate length in A. taiwanensis (9.9+/-0.6 microm) and in Ascogregarina sp. (10.7+/-1.1 microm) isolated from O. j. japonicus. The ultrastructure of the surface of the A. culicis oocyst was rough in texture with numerous dense spots and was easily distinguishable from the oocysts of the other three Ascogregarina spp. The maximum likelihood tree inferred from small subunit ribosomal DNA (SSU rDNA) sequences indicated that the four Ascogregarina spp. form a monophyletic cluster among other gregarine parasites. Within the Ascogregarina clade, A. culicis, A. taiwanensis, and Ascogregarina sp. from O. j. japonicus showed a close relationship, whereas A. armigerei was a distantly related species.
本研究利用扫描电子显微镜对四种分离出的阿氏簇虫（白纹伊蚊体内的台湾阿氏簇虫（连和莱文）（真簇虫科：莱库簇虫科）、埃及伊蚊体内的库蚊阿氏簇虫（罗斯）（真簇虫科：莱库簇虫科）、骚扰阿蚊体内的亚美阿氏簇虫（真簇虫科：莱库簇虫科）以及日本伊蚊体内的阿氏簇虫属物种（真簇虫科：莱库簇虫科））卵囊的超微结构进行了观察，结果显示其在大小和表面结构上存在显著差异。亚美阿氏簇虫卵囊的平均长度最大（13.2±0.2微米）（平均值±标准差），库蚊阿氏簇虫卵囊的平均长度最小（8.8±0.4微米）。台湾阿氏簇虫（9.9±0.6微米）和从日本伊蚊分离出的阿氏簇虫属物种（10.7±1.1微米）的卵囊长度适中。库蚊阿氏簇虫卵囊表面的超微结构质地粗糙，有许多密集的斑点，很容易与其他三种阿氏簇虫的卵囊区分开来。从小亚基核糖体DNA（SSU rDNA）序列推断出的最大似然树表明，这四种阿氏簇虫在其他簇虫寄生虫中形成一个单系类群。在阿氏簇虫分支内，库蚊阿氏簇虫、台湾阿氏簇虫和从日本伊蚊分离出的阿氏簇虫属物种显示出密切的关系，而亚美阿氏簇虫是一个亲缘关系较远的物种。
