Tsg101与马尔堡病毒VP40的相互作用依赖于PPPY基序，而不像埃博拉病毒那样依赖于PT/SAP基序，并且Tsg101在由VP40、NP和GP诱导的马尔堡病毒样颗粒出芽过程中起关键作用。
Interaction of Tsg101 with Marburg virus VP40 depends on the PPPY motif, but not the PT/SAP motif as in the case of Ebola virus, and Tsg101 plays a critical role in the budding of Marburg virus-like particles induced by VP40, NP, and GP.
作者信息
Urata Shuzo, Noda Takeshi, Kawaoka Yoshihiro, Morikawa Shigeru, Yokosawa Hideyoshi, Yasuda Jiro
机构信息
First Department of Forensic Science, National Research Institute of Police Science, Kashiwa 277-0882, Japan.
出版信息
J Virol. 2007 May;81(9):4895-9. doi: 10.1128/JVI.02829-06. Epub 2007 Feb 14.
Abstract
Marburg virus (MARV) VP40 is a matrix protein that can be released from mammalian cells in the form of virus-like particles (VLPs) and contains the PPPY sequence, which is an L-domain motif. Here, we demonstrate that the PPPY motif is important for VP40-induced VLP budding and that VLP production is significantly enhanced by coexpression of NP and GP. We show that Tsg101 interacts with VP40 depending on the presence of the PPPY motif, but not the PT/SAP motif as in the case of Ebola virus, and plays an important role in VLP budding. These findings provide new insights into the mechanism of MARV budding.
摘要
马尔堡病毒（MARV）的VP40是一种基质蛋白，它可以以病毒样颗粒（VLP）的形式从哺乳动物细胞中释放出来，并含有PPPY序列，这是一种L结构域基序。在此，我们证明PPPY基序对VP40诱导的VLP出芽很重要，并且通过共表达NP和GP可显著增强VLP的产生。我们表明，Tsg101根据PPPY基序的存在与VP40相互作用，而不像埃博拉病毒那样依赖于PT/SAP基序，并且在VLP出芽中起重要作用。这些发现为马尔堡病毒出芽机制提供了新的见解。
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