9号染色体长臂34.3区域的杂合性缺失和Notch1基因甲基化较少参与口腔鳞状细胞癌的发生。
LOH at chromosome 9q34.3 and the Notch1 gene methylation are less involved in oral squamous cell carcinomas.
作者信息
Gao Shan, Krogdahl Annelise, Eiberg Hans, Liu Chung-Ji, Sørensen Jens Ahm
机构信息
Department of Molecular Biology, University of Aarhus, Aarhus C, Denmark.
出版信息
J Oral Pathol Med. 2007 Mar;36(3):173-6. doi: 10.1111/j.1600-0714.2007.00520.x.
BACKGROUND
Previous studies of oral carcinomas have shown that both loss of heterozygosity (LOH) and hypermethylation at chromosome 9q33 to 9q34.2 are frequent. The present study investigates the frequency of Notch1 gene methylation and LOH at 9q34.3 region.
METHODS
Gene promoter hypermethylation of the Notch1 gene was analysed by methylation-specific PCR and LOH was analysed using microsatellite markers.
RESULTS
We found LOH at 9q34.3 in three patients and methylation of the Notch1 gene only in two patients with oral carcinoma.
CONCLUSION
Comparing with the alterations at 9q33 to 34.2 regions, LOH at 9q34.3 and methylation of the Notch1 gene was less involved in oral squamous cell carcinomas.
背景
先前关于口腔癌的研究表明，9q33至9q34.2区域的杂合性缺失（LOH）和高甲基化都很常见。本研究调查了Notch1基因甲基化和9q34.3区域LOH的频率。
方法
通过甲基化特异性PCR分析Notch1基因的基因启动子高甲基化，并使用微卫星标记分析LOH。
结果
我们在3例患者中发现9q34.3区域存在LOH，仅在2例口腔癌患者中发现Notch1基因甲基化。
结论
与9q33至34.2区域的改变相比，9q34.3区域的LOH和Notch1基因甲基化在口腔鳞状细胞癌中的参与度较低。
Zotero 插件