Woo Gye-Hyeong, Shibutani Makoto, Inoue Kaoru, Fujimoto Hitoshi, Takahashi Miwa, Lee Kyoung-Youl, Hirose Masao
Division of Pathology, National Institute of Health Sciences, 1-18-1 Kamiyoga, Setagaya-ku, Tokyo 158-8501, Japan.
Food Chem Toxicol. 2007 Jul;45(7):1160-4. doi: 10.1016/j.fct.2006.12.023. Epub 2007 Jan 11.
Jamaica quassia extract (JQE), a natural bittering agent, was investigated for hepatocarcinogenesis-promoting potential using a medium-term liver bioassay system. F344 male rats were given a single intraperitoneal injection of diethylnitrosamine (200mg/kg body weight) and then starting 2 weeks later, received JQE in the diet at concentrations of 500, 5000 or 30,000 ppm for 6 weeks. Animals for tumor promotion (+) and (-) controls were fed 500 ppm sodium phenobarbital (PB) and basal diet, respectively during the promotion phase in this model. All animals were subjected to two-thirds partial hepatectomy at week 3 and killed at week 8. As with the PB-promoted case, both numbers and areas of glutathione S-transferase placental form-positive liver cell foci were significantly increased by JQE at 30,000 ppm, with non-significant increases evident at 5000 ppm. The results thus indicate that JQE at high dose has promoting potential for rat hepatocarcinogenesis.
牙买加苦木提取物(JQE)是一种天然苦味剂,利用中期肝脏生物测定系统对其促肝癌发生的潜力进行了研究。给F344雄性大鼠腹腔注射一次二乙基亚硝胺(200mg/kg体重),然后在2周后开始,在饮食中分别给予浓度为500、5000或30000ppm的JQE,持续6周。在该模型的促进阶段,肿瘤促进(+)和(-)对照组动物分别喂食500ppm苯巴比妥(PB)和基础饮食。所有动物在第3周接受三分之二部分肝切除术,并在第8周处死。与PB促进的情况一样,30000ppm的JQE可使谷胱甘肽S-转移酶胎盘形式阳性肝细胞灶的数量和面积显著增加,5000ppm时增加不明显。因此,结果表明高剂量的JQE具有促进大鼠肝癌发生的潜力。