一氧化氮还原酶在脂多糖激活的巨噬细胞中对铜绿假单胞菌生存的作用。
Participation of nitric oxide reductase in survival of Pseudomonas aeruginosa in LPS-activated macrophages.
作者信息
Kakishima Kohei, Shiratsuchi Akiko, Taoka Azuma, Nakanishi Yoshinobu, Fukumori Yoshihiro
机构信息
Graduate School of Natural Science and Technology, Kanazawa University, Kanazawa, Ishikawa 920-1192, Japan.
出版信息
Biochem Biophys Res Commun. 2007 Apr 6;355(2):587-91. doi: 10.1016/j.bbrc.2007.02.017. Epub 2007 Feb 12.
Nitric oxide (NO) plays a crucial role in the antimicrobial activity of host defense systems. We investigated the function of Pseudomonas aeruginosa NO reductase as a detoxifying enzyme in phagocytes. We found that the growth of the NO reductase-deficient mutant of P. aeruginosa under a microaerobic condition was inhibited by the exogenous NO. Furthermore, the intracellular survival assay within the NO-producing RAW 264.7 macrophages revealed that the wild-type strain survived longer than the NO reductase-deficient mutant. These results suggest that the P. aeruginosa NO reductase may contribute to the intracellular survival by acting as a counter component against the host's defense systems.
一氧化氮（NO）在宿主防御系统的抗菌活性中起着至关重要的作用。我们研究了铜绿假单胞菌NO还原酶作为吞噬细胞中解毒酶的功能。我们发现，在微需氧条件下，铜绿假单胞菌NO还原酶缺陷型突变体的生长受到外源性NO的抑制。此外，在产生NO的RAW 264.7巨噬细胞内的存活试验表明，野生型菌株比NO还原酶缺陷型突变体存活时间更长。这些结果表明，铜绿假单胞菌NO还原酶可能通过作为对抗宿主防御系统的反制成分来促进细胞内存活。
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