Lise Fernanda, Garcia Flávio Roberto Mello, Lutinski Junir Antônio
Departamento de Enfermagem Materno-Infantil, Escola de Enfermagem, Universidade Federal do Rio Grande do Sul, Porto Alegre, RS, Brazil.
Rev Soc Bras Med Trop. 2006 Nov-Dec;39(6):523-6. doi: 10.1590/s0037-86822006000600002.
The objective of this study was to identify ant occurrence in hospital environments in the State of Santa Catarina, along with associated bacteria. Ants were collected monthly from five inpatient clinics in two hospitals in the municipality of Chapecó, from August 2003 to June 2004. They were collected under aseptic conditions using swabs moistened with sterile distilled water and put into test tubes containing BHI for microbiological analysis. After 24 hours, cultures were made in both 5% sheep blood and MacConkey agar, which were incubated for 24 hours at 35/37 degrees C. The Gram characterization, culture identification and biochemical characterization followed standardized rules for clinical microbiology. Seven species of ants were identified, of which the most frequent were Monomorium pharaonis (71.5%) and Solenopsis saevissima (57%), and nineteen species of bacteria was isolated from hospital "A".
本研究的目的是确定圣卡塔琳娜州医院环境中蚂蚁的出现情况以及相关细菌。2003年8月至2004年6月,每月从沙佩科市两家医院的五个住院诊所收集蚂蚁。在无菌条件下,用无菌蒸馏水浸湿的拭子收集蚂蚁,并放入含有脑心浸液肉汤(BHI)的试管中进行微生物分析。24小时后,在5%羊血琼脂和麦康凯琼脂上进行培养,并在35/37摄氏度下孵育24小时。革兰氏鉴定、培养物鉴定和生化鉴定遵循临床微生物学的标准化规则。鉴定出七种蚂蚁,其中最常见的是法老小家蚁(71.5%)和野蛮收获蚁(57%),并且从“A”医院分离出了19种细菌。