Pan X B, Wei L, Chen H S, Liu F, Gao Y
Peking University Hepatology Institute, Peking University People's Hospital, Beijing, P.R. China.
Arch Virol. 2007;152(6):1159-73. doi: 10.1007/s00705-006-0927-4. Epub 2007 Feb 19.
Hepatitis B virus (HBV) infection is currently still a worldwide heath concern. In our study, we compared HBV replication patterns in two liver-derived cell lines, QSG-7701 and HepG2. Viral markers of HBV replication in culture medium and cells were analyzed after transfection of these cells with plasmid pUC18-HBV1.2 into. We showed that QSG-7701 cells could support more stable and a higher level of HBV replication than HepG2 cells. Gene expression profiles of QSG-7701 and HepG2 cells by microarray analysis showed that many genes were differentially expressed between these two cell lines, including those that are related to the HBV life cycle. The global gene expression profile of these two cell types provides some clues to explain how different HBV replication is achieved. QSG-7701 cells offer a new opportunity for basic research on HBV virus-host interactions.
乙型肝炎病毒（HBV）感染目前仍是一个全球健康问题。在我们的研究中，我们比较了两种肝脏来源的细胞系QSG - 7701和HepG2中的HBV复制模式。在用质粒pUC18 - HBV1.2转染这些细胞后，分析了培养基和细胞中HBV复制的病毒标志物。我们发现，QSG - 7701细胞比HepG2细胞能支持更稳定、更高水平的HBV复制。通过微阵列分析QSG - 7701和HepG2细胞的基因表达谱，结果显示这两种细胞系之间有许多基因差异表达，包括那些与HBV生命周期相关的基因。这两种细胞类型的整体基因表达谱为解释如何实现不同的HBV复制提供了一些线索。QSG - 7701细胞为HBV病毒 - 宿主相互作用的基础研究提供了新的机会。
