Krueger Andrew T, Lu Haige, Lee Alex H F, Kool Eric T
Department of Chemistry, Stanford University, Stanford, California 94305, USA.
Acc Chem Res. 2007 Feb;40(2):141-50. doi: 10.1021/ar068200o.
We describe the design, synthesis, and properties of DNA-like molecules in which the base pairs are expanded by benzo homologation. The resulting size-expanded genetic helices are called xDNA ("expanded DNA") and yDNA ("wide DNA"). The large component bases are fluorescent, and they display high stacking affinity. When singly substituted into natural DNA, they are destabilizing because the benzo-expanded base pair size is too large for the natural helix. However, when all base pairs are expanded, xDNA and yDNA form highly stable, sequence-selective double helices. The size-expanded DNAs are candidates for components of new, functioning genetic systems. In addition, the fluorescence of expanded DNA bases makes them potentially useful in probing nucleic acids.
我们描述了碱基对通过苯并同系化而扩展的类DNA分子的设计、合成及性质。由此产生的尺寸扩展的遗传螺旋被称为xDNA(“扩展DNA”)和yDNA(“宽DNA”)。这些较大的组成碱基具有荧光性,并且表现出高堆积亲和力。当单取代进入天然DNA时,它们会使DNA不稳定,因为苯并扩展的碱基对尺寸对于天然螺旋来说太大了。然而,当所有碱基对都被扩展时,xDNA和yDNA会形成高度稳定的、序列选择性的双螺旋。尺寸扩展的DNA是新型功能性遗传系统组件的候选者。此外,扩展DNA碱基的荧光性使其在探测核酸方面具有潜在用途。