Tsujikawa Kenji, Kuwayama Kenji, Miyaguchi Hajime, Kanamori Tatsuyuki, Iwata Yuko, Inoue Hiroyuki, Yoshida Takemi, Kishi Tohru
National Research Institute of Police Science, 6-3-1 Kashiwanoha, Kashiwa, Chiba 277-0882, Japan.
J Chromatogr B Analyt Technol Biomed Life Sci. 2007 Jun 1;852(1-2):430-5. doi: 10.1016/j.jchromb.2007.01.046. Epub 2007 Feb 8.
A reliable analytical method was developed for the quantification and identification of muscimol (MUS) and ibotenic acid (IBO), the toxic constituents of Amanita muscaria and Amanita pantherina. MUS and IBO were extracted from mushrooms by aqueous methanol and derivatized with dansyl chloride (DNS-Cl). After extraction with ethyl acetate and evaporation of the solvent, the residue was ethylated with 1.25 M hydrogen chloride in ethanol. The resulting derivatives were quantified by high-performance liquid chromatography with UV detection and identified by liquid chromatography electrospray ionization tandem mass spectrometry. Calibration curves were linear in the range of 25-2500 ppm for MUS and 40-2500 ppm for IBO, respectively. This method was successfully applied to identify and quantify MUS and IBO in Amanita mushrooms naturally grown and circulated in the drug market.
开发了一种可靠的分析方法，用于定量和鉴定毒蝇伞和豹斑毒伞的有毒成分蝇蕈醇（MUS）和鹅膏蕈氨酸（IBO）。通过甲醇水溶液从蘑菇中提取MUS和IBO，并用丹磺酰氯（DNS-Cl）进行衍生化。用乙酸乙酯萃取并蒸发溶剂后，残留物用1.25 M乙醇中的氯化氢进行乙基化。所得衍生物通过带紫外检测的高效液相色谱法定量，并通过液相色谱电喷雾电离串联质谱法进行鉴定。MUS的校准曲线在25 - 2500 ppm范围内呈线性，IBO的校准曲线在40 - 2500 ppm范围内呈线性。该方法成功应用于鉴定和定量在药物市场自然生长和流通的毒蝇伞属蘑菇中的MUS和IBO。
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