Gu Weiqiong, Li Xiaoying, Liu Changqin, Yang Jun, Ye Lei, Tang Jingfen, Gu Yuanjun, Yang Yisheng, Hong Jie, Zhang Yifei, Chen Mingdao, Ning Guang
Shanghai Clinical Center for Endocrine and Metabolic Diseases, Shanghai Institute of Endocrine and Metabolic Diseases, Rui-Jin Hospital, Shanghai Jiaotong University School of Medicine, PR China.
Endocrine. 2006 Oct;30(2):217-21. doi: 10.1385/ENDO:30:2:217.
Adiponectin plays an important role in improving insulin resistance and preventing atherosclerosis. However it has been rarely reported that adiponectin influences insulin secretion because its receptor was identified in human islet beta cells. In order to investigate the direct effect of adiponectin on pancreatic islet beta cells, we performed an insulin secretion test in purified rat islets, which were incubated with adiponectin (100 ng/mL) at low (3.3 mM) and high (16.7 mM) glucose concentrations. Furthermore, cell lysates were extracted from the adiponectin-treated islets for p-AMPKalpha assay. RTPCR and immunohistochemical examination showed both adiponectin receptor 1 (AdipoR1) and receptor 2 (AdipoR2) were expressed in islet cells and AdipoR1 was predominantly expressed. Insulin secretion was significantly increased in the presence of adiponectin for 6 h at high glucose concentration. Meanwhile, the levels of phosphorylated AMPK increased with adiponectin treatment at high glucose concentrations. It is concluded that adiponectin augments insulin secretion from pancreatic islet beta cells at high glucose concentration through AMPK activation.
脂联素在改善胰岛素抵抗和预防动脉粥样硬化方面发挥着重要作用。然而，由于其受体在人胰岛β细胞中被鉴定出来，脂联素对胰岛素分泌的影响却鲜有报道。为了研究脂联素对胰岛β细胞的直接作用，我们在纯化的大鼠胰岛中进行了胰岛素分泌试验，这些胰岛在低（3.3 mM）和高（16.7 mM）葡萄糖浓度下与脂联素（100 ng/mL）一起孵育。此外，从经脂联素处理的胰岛中提取细胞裂解物用于p-AMPKα检测。逆转录聚合酶链反应（RTPCR）和免疫组织化学检查显示，脂联素受体1（AdipoR1）和受体2（AdipoR2）在胰岛细胞中均有表达，且AdipoR1表达占主导。在高葡萄糖浓度下，脂联素存在6小时时胰岛素分泌显著增加。同时，在高葡萄糖浓度下，随着脂联素处理，磷酸化AMPK水平升高。得出的结论是，脂联素在高葡萄糖浓度下通过激活AMPK增加胰岛β细胞的胰岛素分泌。
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