Mercer D W, Ritchie W P, Dempsey D T
Department of Surgery, Temple University School of Medicine, Philadelphia, Pennsylvania 19140.
Am J Surg. 1992 Jan;163(1):12-7; discussion 17-8. doi: 10.1016/0002-9610(92)90245-m.
Pretreatment with the mild irritant 1 mmol acidified taurocholate protects the gastric mucosa from the injury induced by the subsequent application of 5 mmol acidified taurocholate, a phenomenon referred to as "adaptive cytoprotection." How this occurs remains an enigma. The purpose of this study was to investigate the role of sensory neurons and mucus secretion in this phenomenon. Prior to injury with 5 mmol acidified taurocholate (pH 1.2), the stomachs of six groups of rats were subjected to the following protocol. Two groups were topically pretreated with either saline or the mild irritant 1 mmol acidified taurocholate. Two other groups received the topical anesthetic 1% lidocaine prior to pretreatment with either saline or 1 mmol acidified taurocholate. The last two groups got the mucolytic agent 10% N-acetylcysteine (NAC) after pretreatment with either saline or 1 mmol acidified taurocholate. Injury was assessed by measuring net transmucosal ion fluxes, luminal appearance of deoxyribonucleic acid (DNA), and gross and histologic injury. Pretreatment with the mild irritant 1 mmol acidified taurocholate significantly decreased bile acid-induced luminal ion fluxes and DNA accumulation, suggesting mucosal protection (corroborated by gross and histologic injury analysis). This effect was negated by lidocaine but not by NAC. Thus, it appears that sensory neurons, and not increased mucus secretion, play a critical role in adaptive cytoprotection.
用轻度刺激物1毫摩尔酸化牛磺胆酸盐进行预处理，可保护胃黏膜免受随后应用5毫摩尔酸化牛磺胆酸盐所诱导的损伤，这一现象被称为“适应性细胞保护”。其发生机制仍是一个谜。本研究的目的是探讨感觉神经元和黏液分泌在这一现象中的作用。在用5毫摩尔酸化牛磺胆酸盐（pH 1.2）造成损伤之前，对六组大鼠的胃进行如下处理。两组分别用生理盐水或轻度刺激物1毫摩尔酸化牛磺胆酸盐进行局部预处理。另外两组在分别用生理盐水或1毫摩尔酸化牛磺胆酸盐预处理之前接受局部麻醉剂1%利多卡因。最后两组在分别用生理盐水或1毫摩尔酸化牛磺胆酸盐预处理之后给予黏液溶解剂10%N - 乙酰半胱氨酸（NAC）。通过测量净跨黏膜离子通量、脱氧核糖核酸（DNA）的腔内外观以及大体和组织学损伤来评估损伤情况。用轻度刺激物1毫摩尔酸化牛磺胆酸盐进行预处理可显著降低胆汁酸诱导的腔内离子通量和DNA积累，提示黏膜受到保护（大体和组织学损伤分析证实了这一点）。利多卡因可消除这一效应，但NAC不能。因此，似乎感觉神经元而非黏液分泌增加在适应性细胞保护中起关键作用。
