Martins C F, Rumpf R, Pereira D C, Dode M N
University of Brasília, Department of Cellular Biology, Distrito Federal, Brazil.
Anim Reprod Sci. 2007 Oct;101(3-4):326-31. doi: 10.1016/j.anireprosci.2007.01.018. Epub 2007 Feb 4.
The present study aimed to evaluate viability and in vitro fertilizing ability of cryopreserved epididymal spermatozoa obtained from dead animals. To collect spermatozoa, epididymides from three males (Bulls A1, A2 and A3) were collected at a local slaughterhouse. As a reference ejaculate from a bull with known in vitro fertility, was used. Sperm characteristics (motility, chromatin and acrosome integrity) were evaluated before and after cryopreservation. Then, frozen spermatozoa from all animals were used for in vitro fertilization. Cleavage and blastocyst rates at 48 h (day 2) and 168 h (day 7) post in vitro insemination, for bull A1 (82.1 and 38.6%) and A2 (80.7 and 33.8%) were similar (P>0.05) to the reference bull (88.9 and 57.2%). Bull A3 had the lesser cleavage (42.0%) and blastocyst (26.1%) rates. The results showed that epididymal spermatozoa from dead animals can be successfully cryopreserved and used in vitro production of embryos.
本研究旨在评估从死亡动物获取的冷冻附睾精子的活力和体外受精能力。为收集精子，在当地一家屠宰场采集了三头公牛（公牛A1、A2和A3）的附睾。使用一头已知体外受精能力的公牛的射精作为对照。在冷冻保存前后评估精子特征（活力、染色质和顶体完整性）。然后，将所有动物的冷冻精子用于体外受精。体外受精后48小时（第2天）和168小时（第7天），公牛A1（82.1%和38.6%）和A2（80.7%和33.8%）的卵裂率和囊胚率与对照公牛（88.9%和57.2%）相似（P>0.05）。公牛A3的卵裂率（42.0%）和囊胚率（26.1%）较低。结果表明，从死亡动物获取的附睾精子可以成功冷冻保存并用于体外胚胎生产。
