一个推测的用于氨基阿拉伯糖生物合成的基因簇对洋葱伯克霍尔德菌的生存能力至关重要。
A putative gene cluster for aminoarabinose biosynthesis is essential for Burkholderia cenocepacia viability.
作者信息
Ortega Ximena P, Cardona Silvia T, Brown Alan R, Loutet Slade A, Flannagan Ronald S, Campopiano Dominic J, Govan John R W, Valvano Miguel A
机构信息
Department of Microbiology and Immunology, Infectious Diseases Research Group, Siebens-Drake Medical Research Institute, University of Western Ontario, London, Ontario, Canada.
出版信息
J Bacteriol. 2007 May;189(9):3639-44. doi: 10.1128/JB.00153-07. Epub 2007 Mar 2.
Abstract
Using a conditional mutagenesis strategy we demonstrate here that a gene cluster encoding putative aminoarabinose (Ara4N) biosynthesis enzymes is essential for the viability of Burkholderia cenocepacia. Loss of viability is associated with dramatic changes in bacterial cell morphology and ultrastructure, increased permeability to propidium iodide, and sensitivity to sodium dodecyl sulfate, suggesting a general cell envelope defect caused by the lack of Ara4N.
摘要
利用条件性诱变策略，我们在此证明，一个编码假定氨基阿拉伯糖（Ara4N）生物合成酶的基因簇对洋葱伯克霍尔德菌的生存能力至关重要。生存能力的丧失与细菌细胞形态和超微结构的显著变化、对碘化丙啶的通透性增加以及对十二烷基硫酸钠的敏感性有关，这表明缺乏Ara4N会导致一般的细胞包膜缺陷。
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