Zhang Dan, Boulware Michael J, Pendleton Matthew R, Nogi Taisaku, Marchant Jonathan S
Department of Pharmacology, University of Minnesota Medical School, MN 55455, USA.
Biochem J. 2007 Jun 15;404(3):383-91. doi: 10.1042/BJ20070101.
Studies in the Xenopus model system have provided considerable insight into the developmental role of intracellular Ca2+ signals produced by activation of IP3Rs (inositol 1,4,5-trisphosphate receptors). However, unlike mammalian systems where three IP3R subtypes have been well characterized, our molecular understanding of the IP3Rs that underpin Ca2+ signalling during Xenopus embryogenesis relate solely to the original characterization of the 'Xenopus IP3R' cloned and purified from Xenopus laevis oocytes several years ago. In the present study, we have identified Xenopus type 2 and type 3 IP3Rs and report the full-length sequence, genomic architecture and developmental expression profile of these additional IP3R subtypes. In the light of the emerging genomic resources and opportunities for genetic manipulation in the diploid frog Xenopus tropicalis, these data will facilitate manipulations to resolve the contribution of IP3R diversity in Ca2+ signalling events observed during vertebrate development.
非洲爪蟾模型系统的研究为深入了解由1,4,5-三磷酸肌醇受体（IP3Rs）激活所产生的细胞内Ca2+信号在发育过程中的作用提供了大量信息。然而，与已对三种IP3R亚型进行充分表征的哺乳动物系统不同，我们对非洲爪蟾胚胎发育过程中支撑Ca2+信号传导的IP3Rs的分子理解仅基于数年前从非洲爪蟾卵母细胞中克隆和纯化的“非洲爪蟾IP3R”的最初表征。在本研究中，我们鉴定出了非洲爪蟾2型和3型IP3Rs，并报告了这些额外IP3R亚型的全长序列、基因组结构和发育表达谱。鉴于二倍体青蛙热带爪蟾中不断涌现的基因组资源和基因操作机会，这些数据将有助于进行操作，以解析IP3R多样性在脊椎动物发育过程中所观察到的Ca2+信号传导事件中的作用。
