血浆瘦素水平与身体组成、性别、胰岛素水平以及解偶联蛋白2基因的A55V多态性有关。

Plasma leptin levels are related to body composition, sex, insulin levels and the A55V polymorphism of the UCP2 gene.

作者信息

Rance K A, Johnstone A M, Murison S, Duncan J S, Wood S G, Speakman J R

机构信息

Aberdeen Centre for Energy Regulation and Obesity (ACERO), Rowett Research Institute, Bucksburn, Aberdeen, Scotland, UK.

出版信息

Int J Obes (Lond). 2007 Aug;31(8):1311-8. doi: 10.1038/sj.ijo.0803535. Epub 2007 Mar 6.

DOI:10.1038/sj.ijo.0803535

PMID:17342078

Abstract

OBJECTIVE

Circulating leptin levels show a high degree of individual variability even after the main effect of body fatness is accounted for. We therefore wanted to determine the roles of variation in body composition, age, sex and polymorphisms of the UCP2 gene and promoter region on levels of circulating leptin.

SUBJECTS

One hundred and fifty Caucasian subjects, which represented a cross-section of the population from NE, Scotland, were recruited.

MEASUREMENTS

Body composition was measured using dual X-ray absorptiometry. Fasted circulating leptin, insulin, T3 and T4 levels were measured, and all individuals were genotyped for the UCP2 polymorphisms A55V, -866G>A and exon-8 ins/del.

RESULTS

The results indicate that circulating leptin was significantly related to sex and principle component (PC) scores representing overall adipose tissue mass and a second representing the contrast of central to peripheral bone mineral content. Residual leptin was associated with the A55V polymorphism (P< 0.001) explaining 11.3% of the residual variance. There was a marginal effect associated with exon-8 ins/del (P=0.045) explaining 4.4% of the residual variance in leptin. Log(e) transformed circulating fasting insulin was related to PC scores representing general adiposity and sex. Residual Log(e) insulin was associated with the A55V and exon-8 ins/del polymorphisms explaining 5.7% (P=0.015) and 5% (P=0.026) of the residual variation, respectively. The -866G>A polymorphism was not significantly associated with residual leptin or insulin. Leptin and insulin were significantly (P=0.007) correlated. Statistically removing the effect of insulin on leptin still showed association between leptin and A55V (P=0.002). Removing the effect of leptin on insulin, the A55V polymorphism was no longer significant (P=0.120). After accounting for the correlation between insulin and leptin, the exon-8 ins/del was no longer significant for residual leptin (P=0.119) or Log(e) insulin (P=0.252).

CONCLUSION

These data suggest that the A55V polymorphism directly affected the levels of leptin but not via an effect on insulin.

摘要

目的

即使在考虑了体脂的主要影响因素之后，循环瘦素水平仍表现出高度的个体差异。因此，我们希望确定身体成分、年龄、性别以及UCP2基因和启动子区域多态性的变化对循环瘦素水平的作用。

受试者

招募了150名白种人受试者，他们代表了来自苏格兰东北部人群的一个横断面。

测量

使用双能X线吸收法测量身体成分。测量空腹循环瘦素、胰岛素、T3和T4水平，并对所有个体进行UCP2多态性A55V、-866G>A和外显子8插入/缺失的基因分型。

结果

结果表明，循环瘦素与性别以及代表总体脂肪组织量的主成分（PC）得分和代表中央与外周骨矿物质含量对比的第二个PC得分显著相关。残余瘦素与A55V多态性相关（P<0.001），解释了11.3%的残余变异。外显子8插入/缺失存在边缘效应（P=0.045），解释了瘦素4.4%的残余变异。对数（e）转换后的空腹循环胰岛素与代表总体肥胖和性别的PC得分相关。残余对数（e）胰岛素与A55V和外显子8插入/缺失多态性相关，分别解释了5.7%（P=0.015）和5%（P=0.026）的残余变异。-866G>A多态性与残余瘦素或胰岛素无显著相关性。瘦素和胰岛素显著相关（P=0.007）。从统计学上消除胰岛素对瘦素的影响后，瘦素与A55V之间仍存在关联（P=0.002）。消除瘦素对胰岛素的影响后，A55V多态性不再显著（P=0.120）。考虑胰岛素与瘦素之间的相关性后，外显子8插入/缺失对残余瘦素（P=0.119）或对数（e）胰岛素（P=0.252）不再显著。