Shi Qi, Wang Yong-Jun, Li Chen-Guang
Institute of Spinal Disease, Longhua Hospital, Shanghai University of TCM, Shanghai.
Zhongguo Zhong Xi Yi Jie He Za Zhi. 2007 Feb;27(2):142-6.
To study the effects of Yiqi Huayu Bushen Recipe (YHBR) and its disassembled recipes on mRNA expressions of collagen I, III, X, matrix metalloproteinase (MMPs) and tissue inhibitor of metalloproteinase (TIMP) in extracellular matrix of cervical disc in model rats of cervical vertebral disc degeneration.
The mRNA expressions of collagens, MMP-13 and TIMP-1 were detected by RT-PCR. The strips were scanned by gel imaging system scanner, and the optical density was autocalculated by computer.
Compared with those of the sham-operative group, the mRNA expressions of collagen I , Ill and X and MMP-13 of the model rats increased markedly (P < 0.01), which were lowered by YHBR and its disassembled recipes (P < 0.01 or P < 0.05), and the levels after YHBR treatment were significantly different to those after Western medicine treatment. However, no remarkable change was found in TIMP-1 mRNA expression in the model rats (P > 0.05).
In the degenerated intervertebral disc the mRNA expressions of collagen I , III, X and MMP-13 increased, TIMP-1 mRNA expression decreased and the proportion of MMPs/TIMP was in imbalance. YHBR and its disassembled recipes could postpone the degeneration of intervertebral disc through regulating mRNA expressions of collagens and their correlated metabolic enzymes.
研究益气化瘀补肾方（YHBR）及其拆方对颈椎间盘退变模型大鼠颈椎间盘细胞外基质中Ⅰ、Ⅲ、Ⅹ型胶原蛋白、基质金属蛋白酶（MMPs）及基质金属蛋白酶组织抑制剂（TIMP）mRNA表达的影响。
采用RT-PCR法检测胶原蛋白、MMP-13及TIMP-1的mRNA表达。用凝胶成像系统扫描仪扫描条带，计算机自动计算光密度。
与假手术组比较，模型大鼠Ⅰ、Ⅲ、Ⅹ型胶原蛋白及MMP-13的mRNA表达明显升高（P<0.01），YHBR及其拆方可使其降低（P<0.01或P<0.05），且YHBR组治疗后的水平与西药组治疗后的水平有显著差异。然而，模型大鼠TIMP-1 mRNA表达无明显变化（P>0.05）。
在退变椎间盘中，Ⅰ、Ⅲ、Ⅹ型胶原蛋白及MMP-13的mRNA表达升高，TIMP-1 mRNA表达降低，MMPs/TIMP比例失衡。YHBR及其拆方可通过调节胶原蛋白及其相关代谢酶的mRNA表达延缓椎间盘退变。
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