猪（野猪）ADSS1和ADSS2基因的比较分子特征分析
Comparative molecular characterization of ADSS1 and ADSS2 genes in pig (Sus scrofa).
作者信息
Li Xinyun, Zhu Zhengmao, Mo Delin, Wang Heng, Yang Shulin, Zhao Shuhong, Li Kui
机构信息
Department of Gene and Cell Engineering, Institute of Animal Science, Chinese Academy of Agricultural Sciences, Beijing 100094, PR China.
出版信息
Comp Biochem Physiol B Biochem Mol Biol. 2007 Jun;147(2):271-7. doi: 10.1016/j.cbpb.2007.01.013. Epub 2007 Feb 1.
Adenylosuccinate synthetase (ADSS) catalyzes the key step of AMP synthesis. Vertebrates have two isozymes of ADSS, which are named ADSS1 and ADSS2, respectively. In this study, we cloned porcine ADSS1 and ADSS2 genes and comparatively analyzed their sequence, chromosome mapping, mRNA distribution and subcellular localization. According to our results, the ADSS1 gene was predominantly expressed in the striated muscle tissues, while ADSS2 gene distributed widely in all the tissues detected. Additionally, ADSS1 gene was up-regulated significantly along with porcine muscle growth, and ADSS2 gene expression was more constant during the muscle development. Porcine ADSS1 gene was assigned to SSC7q and the linked marker was SSC12B09, ADSS2 gene was mapped on SSC10p and the linked marker was SW497, and porcine ADSS2 protein was subcellular localized in mitochondria. Moreover, we found that one single nucleotide polymorphism (SNP, T/C(70)) in the ninth intron of ADSS2 gene was significantly associated with average daily gain trait (ADG, P<0.05) and loin muscle area trait (P<0.05).
腺苷酸琥珀酸合成酶（ADSS）催化AMP合成的关键步骤。脊椎动物有两种ADSS同工酶，分别命名为ADSS1和ADSS2。在本研究中，我们克隆了猪ADSS1和ADSS2基因，并对它们的序列、染色体定位、mRNA分布和亚细胞定位进行了比较分析。根据我们的结果，ADSS1基因主要在横纹肌组织中表达，而ADSS2基因在所有检测的组织中广泛分布。此外，随着猪肌肉生长，ADSS1基因显著上调，而ADSS2基因在肌肉发育过程中的表达更稳定。猪ADSS1基因定位于SSC7q，连锁标记为SSC12B09，ADSS2基因定位于SSC10p，连锁标记为SW497，猪ADSS2蛋白亚细胞定位于线粒体。此外，我们发现ADSS2基因第九内含子中的一个单核苷酸多态性（SNP，T/C(70)）与平均日增重性状（ADG，P<0.05）和腰大肌面积性状（P<0.05）显著相关。
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