Chapman C, Murray A, Chakrabarti J, Thorpe A, Woolston C, Sahin U, Barnes A, Robertson J
Division of Breast Surgery, The University of Nottingham, Nottingham, UK.
Ann Oncol. 2007 May;18(5):868-73. doi: 10.1093/annonc/mdm007. Epub 2007 Mar 7.
There is increasing evidence that the immune system produces a humoral response to cancer-derived antigens. This study assessed the diagnostic potential of autoantibodies to multiple known tumour-associated proteins.
Sera from normal controls (n = 94), primary breast cancer patients (n = 97) and patients with ductal carcinoma in situ (DCIS) (n = 40) were investigated for the presence of autoantibodies to p53, c-myc, HER2, NY-ESO-1, BRCA1, BRCA2 and MUC1 antigens by enzyme-linked immunosorbent assay.
Reproducibly elevated levels of autoantibodies were seen in at least one of the six antigens in 64% of primary breast cancer patient sera and 45% of patients with DCIS at a specificity of 85%. No significant differences were seen when patients were subdivided by age, tumour size, histological grade, lymph node status or detection methodology.
Autoantibodies against one or more of these tumour-associated antigens appears to indicate the presence of early-stage breast cancers. Autoantibody assays against a panel of antigens could be used as an aid to mammography in the detection and diagnosis of early primary breast cancer, especially in younger women at increased risk of breast cancer where mammography is known to have reduced sensitivity and specificity.
越来越多的证据表明，免疫系统会对癌症衍生抗原产生体液免疫反应。本研究评估了针对多种已知肿瘤相关蛋白的自身抗体的诊断潜力。
通过酶联免疫吸附测定法，检测了正常对照者（n = 94）、原发性乳腺癌患者（n = 97）和原位导管癌（DCIS）患者（n = 40）血清中针对p53、c-myc、HER2、NY-ESO-1、BRCA1、BRCA2和MUC1抗原的自身抗体。
在原发性乳腺癌患者血清中，64%的样本至少有一种自身抗体水平在六种抗原中的一种升高，在DCIS患者中这一比例为45%，特异性为85%。根据年龄、肿瘤大小、组织学分级、淋巴结状态或检测方法对患者进行细分时，未发现显著差异。
针对这些肿瘤相关抗原中一种或多种的自身抗体似乎表明早期乳腺癌的存在。针对一组抗原的自身抗体检测可作为乳房X线摄影的辅助手段，用于早期原发性乳腺癌的检测和诊断，尤其是在乳腺癌风险增加的年轻女性中，已知乳房X线摄影的敏感性和特异性会降低。
