Ewis Ashraf A, El-Samman Essam, Ali Nermin, Kajimoto Kazuaki, Shinohara Yasuo, Ishikawa Mitsuru, Kanayama Hiro-Omi, Baba Yoshinobu
Health Technology Research Center, National Institute of Advanced Industrial Science and Technology Shikoku, Takamatsu, Japan.
Urol Oncol. 2007 Mar-Apr;25(2):120-7. doi: 10.1016/j.urolonc.2006.03.006.
To date, molecular evidence studies for bladder cancer, using the microarray technology, are focusing on the transitional cell carcinoma, however, similar fingerprinting studies have rarely been performed on the other molecular phenotypes of bladder cancer, squamous cell carcinoma (SCC). This study was conducted to monitor the gene expression profiles for bilharzial-related SCC of the bladder to be able to compare its data with transitional cell carcinoma microarray data. A total of 17 paired bilharzial urinary bladder SCC specimens and their corresponding normal urothelium were analyzed using the complementary deoxyribonucleic acid microarray hybridization approach to study the molecular basis of the development of SCC of the urinary bladder. Validation of the microarray results was performed using the Northern blotting technique. After supervised analysis of the microarray data, there was at least a 3-fold difference in the expression between SCC of the bladder and normal urothelium in 82 genes. A total of 38 genes were up-regulated in SCC of the bladder, including matrix degradation-related genes, growth factors, different oncogenes, and immunology related genes. Conversely, 44 genes were down-regulated in SCC of the bladder, including integrins, laminins, cadherins, nonmetastatic cell 1 (NM23) and apoptosis-related genes. Our findings can explain the aggressive behavior of SCC of the bladder. Such gene profiling studies will add to our understanding of the mechanisms of carcinogenesis, and may also improve our ability to diagnose and treat bladder cancer.
迄今为止，利用微阵列技术对膀胱癌进行的分子证据研究主要集中在移行细胞癌上，然而，针对膀胱癌的其他分子表型——鳞状细胞癌（SCC），类似的指纹图谱研究却很少进行。本研究旨在监测膀胱血吸虫病相关鳞状细胞癌的基因表达谱，以便能够将其数据与移行细胞癌微阵列数据进行比较。使用互补脱氧核糖核酸微阵列杂交方法，对总共17对膀胱血吸虫病鳞状细胞癌标本及其相应的正常尿路上皮进行分析，以研究膀胱鳞状细胞癌发生发展的分子基础。使用Northern印迹技术对微阵列结果进行验证。在对微阵列数据进行监督分析后，82个基因在膀胱鳞状细胞癌和正常尿路上皮之间的表达差异至少为3倍。共有38个基因在膀胱鳞状细胞癌中上调，包括与基质降解相关的基因、生长因子、不同的癌基因和免疫相关基因。相反，44个基因在膀胱鳞状细胞癌中下调，包括整合素、层粘连蛋白、钙黏着蛋白、非转移细胞1（NM23）和凋亡相关基因。我们的研究结果可以解释膀胱鳞状细胞癌的侵袭性行为。此类基因谱研究将增进我们对致癌机制的理解，也可能提高我们诊断和治疗膀胱癌的能力。
