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高速逆流色谱法在从新鲜菠菜中分离9'-顺式新黄质中的应用。

Application of high-speed counter-current chromatography for the isolation of 9'-cis-neoxanthin from fresh spinach.

作者信息

Baldermann Susanne, Reinhard Annika, Köhler Nils, Fleischmann Peter

机构信息

Institute of Food Chemistry, Technical University of Braunschweig, Schleinitzstrasse 20, 38106 Braunschweig, Germany.

出版信息

J Chromatogr A. 2007 Jun 1;1151(1-2):183-6. doi: 10.1016/j.chroma.2007.02.064. Epub 2007 Feb 24.

DOI:10.1016/j.chroma.2007.02.064
PMID:17350636
Abstract

Preparative HSCCC (high-speed counter-current chromatography) could be applied for the isolation of 9'-cis-neoxanthin from a crude carotenoid extract of fresh spinach leaves. The separation was performed on a Pharma-Tech Research Corp. CCC 1000 with a solvent system composed of hexane:ethanol:water at a volume ratio of 5:5:4.5 at a flow rate of 3 mL/min and at 850 rpm, using the lower phase as mobile phase. 9'-cis-neoxanthin with a purity of up to 94% could be obtained with a single HSCCC purification step of the crude carotenoid extract.

摘要

制备型高速逆流色谱（HSCCC）可用于从新鲜菠菜叶的粗类胡萝卜素提取物中分离9'-顺式新黄质。分离在Pharma-Tech Research Corp. CCC 1000上进行，溶剂系统由己烷：乙醇：水按体积比5:5:4.5组成，流速为3 mL/min，转速为850 rpm，使用下层作为流动相。通过对粗类胡萝卜素提取物进行单次HSCCC纯化步骤，可获得纯度高达94%的9'-顺式新黄质。

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