通过单步测定法区分人靶器官提取物中的类固醇激素受体CBG和SHBG。

Differentiation between steroid hormone receptors CBG and SHBG in human target organ extracts by a single-step assay.

作者信息

Wagner R K, Jungblut P W

出版信息

Mol Cell Endocrinol. 1975;4(1):13-24. doi: 10.1016/0303-7207(76)90003-4.

Abstract

The steroid complexes of (plasma) corticosteroid-binding globulin can be distinguished from intracellular steroid-receptor complexes by agar electrophoresis at low temperature in neuraminidase-treated tissue extracts. With this method, the presence of progesterone receptor has been demonstrated in heavily plasma-protein-contaminated human uterus "cytosol", but not in human mammary carcinoma extracts. SHBG and "basic" receptors for estradiol and dihydrotestosterone in human uterus cytosol could also be assayed simultaneously.

摘要

在经神经氨酸酶处理的组织提取物中，通过低温琼脂电泳可将（血浆）皮质类固醇结合球蛋白的类固醇复合物与细胞内类固醇受体复合物区分开来。用这种方法，已证实在严重受血浆蛋白污染的人子宫“胞质溶胶”中存在孕酮受体，但在人乳腺癌提取物中未发现。人子宫胞质溶胶中雌二醇和双氢睾酮的性激素结合球蛋白（SHBG）及“碱性”受体也可同时进行检测。