基因多态性影响卵形疟原虫PCR检测准确性。
Genetic polymorphisms influence Plasmodium ovale PCR detection accuracy.
作者信息
Calderaro A, Piccolo G, Perandin F, Gorrini C, Peruzzi S, Zuelli C, Ricci L, Manca N, Dettori G, Chezzi C, Snounou G
机构信息
Faculty of Medicine and Surgery, University of Parma, Department of Pathology and Laboratory Medicine, Section of Microbiology, Viale A. Gramsci, 14-43100 Parma, Italy.
出版信息
J Clin Microbiol. 2007 May;45(5):1624-7. doi: 10.1128/JCM.02316-06. Epub 2007 Mar 14.
Detection of Plasmodium ovale by use of a nested PCR assay with a novel Plasmodium ovale primer set was superior to detection of Plasmodium ovale by real-time PCR assays. Nested PCR was also better at detecting P. malariae. The detection of P. ovale in many patients first admitted >2 months following their return to Italy indicated that P. ovale relapses are common.
使用带有新型卵形疟原虫引物组的巢式PCR检测法检测卵形疟原虫，比实时PCR检测法检测卵形疟原虫更具优势。巢式PCR在检测三日疟原虫方面也表现更佳。许多首次在返回意大利2个月后才入院的患者中检测到卵形疟原虫，这表明卵形疟原虫复发很常见。
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