Cetin Demet, Dönmez Sedat, Dönmez Gönül
Department of Biology, Faculty of Science, Ankara University, 06100 Beşevler, Ankara, Turkey.
J Environ Manage. 2008 Jul;88(1):76-82. doi: 10.1016/j.jenvman.2007.01.019. Epub 2007 Mar 23.
Sulfate-reducing bacteria (SRB) that could grow on modified Postgate C medium (PC) containing chromium(VI) were isolated from industrial wastewaters and their chromium(VI) reduction capacities were investigated as a function of changes in the initial pH values, chromium, sulfate, NaCl and reactive dye concentrations. The optimum pH value at 50 mg l(-1) initial chromium(VI) concentration was determined to be 8. Chromium(VI) reduction by SRB was investigated at 22.7-98.4 mg l(-1) initial chromium(VI) concentrations. At the end of the experiments, the mixed cultures of SRB were found to reduce within 2-6 days more than 99% of the initial chromium(VI) levels, which ranged from 22.7 to 74.9 mg l(-1). The effects of the initial 0-9.0 g l(-1) concentrations of disodium sulfate and 0-6% (w/v) concentrations of NaCI to chromium reduction showed that the lowest concentrations of sulfate and NaCI were the best for chromium reduction in the PC medium including 50 mg l(-1) chromium(VI). Chromium(VI) reduction in 50 mg l(-1) and 25-100 mg l(-1) Remazol Blue, Reactive Black B or Reactive Red RB containing media were also investigated. In the experiments, 25-30% of the initial dye concentrations and 95% of the chromium(VI) was removed from the medium at the end of 72-h and 24-h incubation periods, respectively.
从工业废水中分离出了能在含有六价铬的改良波斯特盖特C培养基(PC)上生长的硫酸盐还原菌(SRB),并研究了它们的六价铬还原能力与初始pH值、铬、硫酸盐、氯化钠和活性染料浓度变化的关系。在初始六价铬浓度为50 mg l(-1)时,确定最佳pH值为8。在初始六价铬浓度为22.7 - 98.4 mg l(-1)的条件下研究了SRB对六价铬的还原情况。实验结束时,发现SRB混合培养物在2 - 6天内将初始六价铬水平(范围为22.7至74.9 mg l(-1))降低了99%以上。初始浓度为0 - 9.0 g l(-1)的硫酸钠和0 - 6%(w/v)的氯化钠对铬还原的影响表明,在含有50 mg l(-1)六价铬的PC培养基中,最低浓度的硫酸盐和氯化钠最有利于铬的还原。还研究了在含有50 mg l(-1)以及25 - 100 mg l(-1)雷马素蓝、活性黑B或活性红RB的培养基中六价铬的还原情况。在实验中,分别在72小时和24小时的培养期结束时,从培养基中去除了25 - 30%的初始染料浓度和95%的六价铬。