跨损伤合成：Y家族聚合酶与聚合酶转换

Translesion synthesis: Y-family polymerases and the polymerase switch.

作者信息

Lehmann Alan R, Niimi Atsuko, Ogi Tomoo, Brown Stephanie, Sabbioneda Simone, Wing Jonathan F, Kannouche Patricia L, Green Catherine M

机构信息

Genome Damage and Stability Centre, University of Sussex, Falmer, Brighton BN1 9RQ, UK.

出版信息

DNA Repair (Amst). 2007 Jul 1;6(7):891-9. doi: 10.1016/j.dnarep.2007.02.003. Epub 2007 Mar 23.

DOI:10.1016/j.dnarep.2007.02.003

PMID:17363342

Abstract

Replicative DNA polymerases are blocked at DNA lesions. Synthesis past DNA damage requires the replacement of the replicative polymerase by one of a group of specialised translesion synthesis (TLS) polymerases, most of which belong to the Y-family. Each of these has different substrate specificities for different types of damage. In eukaryotes mono-ubiquitination of PCNA plays a crucial role in the switch from replicative to TLS polymerases at stalled forks. All the Y-family polymerases have ubiquitin binding sites that increase their binding affinity for ubiquitinated PCNA at the sites of stalled forks.

摘要

复制性DNA聚合酶会被DNA损伤阻断。跨越DNA损伤进行合成需要用一组特殊的跨损伤合成（TLS）聚合酶中的一种来取代复制性聚合酶，其中大多数属于Y家族。这些聚合酶中的每一种对不同类型的损伤都有不同的底物特异性。在真核生物中，增殖细胞核抗原（PCNA）的单泛素化在停滞叉处从复制性聚合酶向TLS聚合酶的转换中起关键作用。所有Y家族聚合酶都有泛素结合位点，这些位点会增加它们在停滞叉位点对泛素化PCNA的结合亲和力。