采用柱前衍生化法高效液相色谱测定犬血浆中新型有机硒化合物1,2-[双(1,2-苯并异硒唑啉-3(2H)-酮)]-乙烷（BBSKE）及其在药代动力学研究中的应用
High performance liquid chromatographic determination of 1,2-[bis(1,2-benzisoselenazolone-3(2H)-ketone)]-ethane (BBSKE), a novel organoselenium compound, in dog plasma using pre-column derivatization and its application in pharmacokinetic study.
作者信息
Zhou Hai-Yan, Dou Gui-Fang, Meng Zhi-Yun, Lou Ya-Qing, Zhang Guo-Liang
机构信息
Department of Pharmacology, Basic Medical School, Beijing University, Beijing 100083, PR China.
出版信息
J Chromatogr B Analyt Technol Biomed Life Sci. 2007 Jun 1;852(1-2):617-24. doi: 10.1016/j.jchromb.2007.02.040. Epub 2007 Feb 28.
A novel HPLC-UV method with pre-column derivatization by using 2-mercaptoethanol was established for determination of 1,2-[bis(1,2-benzisoselenazolone-3(2H)-ketone)]-ethane (BBSKE) in dog plasma. The derivatives were identified by mass spectrometry. The method had a good linear range of 0.05-2 microg/ml (r(2)=0.9995). The lower limit of quantification (LOQ) was 0.05 microg/ml. The precision and accuracy were less than 7%. After dosing of BBSKE (30 mg/kg, p.o. and 0.79 mg/kg, i.v.) in dogs, AUC(0-t) were 5.72+/-2.42 and 1.35+/-0.41 microg h/ml; t(1/2) were 4.6+/-2.1 and 1.7+/-0.6h, respectively. The method was successfully applied to the pharmacokinetic study in dogs.
建立了一种采用2-巯基乙醇进行柱前衍生化的新型高效液相色谱-紫外检测法，用于测定犬血浆中的1,2-[双(1,2-苯并异硒唑啉-3(2H)-酮)]-乙烷（BBSKE）。通过质谱对衍生物进行鉴定。该方法的线性范围良好，为0.05 - 2微克/毫升（r(2)=0.9995）。定量下限（LOQ）为0.05微克/毫升。精密度和准确度均小于7%。犬口服和静脉注射BBSKE（分别为30毫克/千克和0.79毫克/千克）后，AUC(0-t)分别为5.72±2.42和1.35±0.41微克·小时/毫升；t(1/2)分别为4.6±2.1和1.7±0.6小时。该方法成功应用于犬的药代动力学研究。
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