The aim of this study was to determine reference peak-to-peak threshold sound pressure levels for air conduction sound transducers using groups of tone bursts as test signals. For this purpose, threshold measurements with five different earphones and a loudspeaker were carried out on groups of 25 young, otologically normal test subjects in the frequency range between 250 Hz and 8000 Hz, following as closely as possible the ISO Preferred Test Conditions. The dependence of the results on repetition rate, type of sound transducer, gender and age of the test subjects, and on reference pure tone thresholds was investigated. The results mainly depend on the reference equivalent sound pressure levels for pure tones of each sound transducer. Together with the results of another study carried out in Denmark, the data of the present study will form the basis for the International Standard ISO 389 Part 6 on reference hearing thresholds for acoustic test signals of short duration.