Ashfaq Muhammad, Sonoda Shoji, Tsumuki Hisaaki
Research Institute for Bioresources, Okayama University, Kurashiki, Okayama, Japan.
Arch Insect Biochem Physiol. 2007 Apr;64(4):175-85. doi: 10.1002/arch.20168.
We cloned and characterized two hexameric storage protein genes, PxAry1 and PxAry2, from Plutella xylostella and investigated the expression pattern in different developmental stages and in response to treatment by a juvenile hormone (JH) analog. The complete coding sequences of PxAry1 and PxAry2 are comprised of 2,097 and 2,094 bp with 699 and 698 amino acid residues, respectively. Signal peptides of 16 amino acids are predicted at the N-termini. According to both the phylogenetic analysis and amino acid composition (>16% aromatic amino acids), PxAry1 and PxAry2 belong to the arylphorin-like protein genes. Analysis using Northern hybridization and RT-PCR showed varying levels of genes expression in the developmental stages with a small difference between sexes. Expression of both genes in fourth instar larvae was suppressed after treatment with a JH-analog. Southern hybridization revealed the presence of multiple arylphorin genes in the genome.
我们从小菜蛾中克隆并鉴定了两个六聚体储存蛋白基因PxAry1和PxAry2，并研究了它们在不同发育阶段的表达模式以及对保幼激素（JH）类似物处理的响应。PxAry1和PxAry2的完整编码序列分别由2097和2094个碱基对组成，分别具有699和698个氨基酸残基。在N端预测有16个氨基酸的信号肽。根据系统发育分析和氨基酸组成（>16%芳香族氨基酸），PxAry1和PxAry2属于类芳基脂蛋白基因。使用Northern杂交和RT-PCR分析表明，这些基因在发育阶段的表达水平各不相同，两性之间存在微小差异。用JH类似物处理后，四龄幼虫中这两个基因的表达均受到抑制。Southern杂交显示基因组中存在多个芳基脂蛋白基因。
