Li Gang, Li Xiang-Ping, Liu Xiong, Peng Ying, Lin Marie C
Department of Otolaryngology-Head and Neck Surgery, Nanfang Hospital, Southern Medical University, Guangzhou 510515, China.
Zhonghua Zhong Liu Za Zhi. 2006 Oct;28(10):724-7.
To evaluate the application of DNA vector-based RNA interference for silencing LMP-1 expression in EBV-positive nasopharyngeal cancer (NPC) C611 cells and analyze the inhibiting effects of LMP-1 silencing on metastatic property of NPC cells.
DNA vectors with LMP-1 shRNA template were transfected into C611 cells. After G418 selection, a LMP-1 silenced sub-clone named C611L was isolated. Effect of LMP-1 inhibition on its metastatic properties was evaluated by cell adhesion, invasion and wound healing assays.
shRNA vector mediated stable RNAi against LMP-1 in C611 cells. Loss of LMP-1 significantly altered cell motility, substratum adhesion and transmembrane invasion ability.
Stable suppression of LMP-1 induced by shRNA vector can inhibit metastatic properties of NPC cells.
评估基于DNA载体的RNA干扰技术在EB病毒阳性鼻咽癌（NPC）C611细胞中沉默LMP-1表达的应用，并分析LMP-1沉默对NPC细胞转移特性的抑制作用。
将携带LMP-1 shRNA模板的DNA载体转染至C611细胞。经G418筛选后，分离出一个LMP-1沉默的亚克隆，命名为C611L。通过细胞黏附、侵袭和伤口愈合试验评估LMP-1抑制对其转移特性的影响。
shRNA载体介导C611细胞中对LMP-1的稳定RNA干扰。LMP-1的缺失显著改变了细胞运动性、基质黏附及跨膜侵袭能力。
shRNA载体诱导的LMP-1稳定抑制可抑制NPC细胞的转移特性。
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