热休克蛋白在食管鳞状细胞癌中的表达及意义

[Expression and significance of heat shock proteins in esophageal squamous cell carcinoma].

作者信息

Chen Jun-Hui, Chen Li-Ming, Xu Li-Yan, Wu Ming-Yao, Shen Zhong-Ying

机构信息

Department of Oncology, First Affiliated Hospital of Shantou University Medical College, Guangdong Shantou 515041, China.

出版信息

Zhonghua Zhong Liu Za Zhi. 2006 Oct;28(10):758-61.

PMID:17366788

Abstract

OBJECTIVE

To investigate the expression and significance of HSP27, HSP60, HSP70 and HSP90 alpha in esophageal squamous cell carcinoma (ESCC) and tissues along the incision margin (TIM).

METHODS

The presence and the level of expression of HSP27, HSP60, HSP70 and HSP90 alpha were determined in 168 specimens from ESCC and 42 from tissues along TIM by EnVision immunohistochemistry and Western blotting, to compare their positive staining rates and explore the correlation between their expressions and clinicopathologic features in ESCC.

RESULTS

The positive staining rates of HSP27, HSP60, HSP70 and HSP90 alpha in ESCC and TIM were 62.0% and 42.1%, 92.7% and 63.2%, 57.9% and 22.2%, and 33.7% and 18.5%, respectively. There was very significant difference between the expression of HSP60 and HSP70 in ESCC and TIM (P < 0.01), but not significant about HSP27 and HSP90 alpha (P > 0.05). The positive staining rate of HSP27 declined with the lower grade of differentiation of ESCC (P < 0.05).

CONCLUSION

The present findings suggest that the expression of HSPs of different molecular weight in ESCC and TIM is a common event. The level of expressions of HSP60 and HSP70 are higher than those in TIM. HSP60 and HSP70 expression correlated with the biological behavior of ESCC. The expression of HSP27 was positively correlated to the grade of differentiation of ESCC. Overexpression of HSP27 may be associated to the differentiation of squamous cell carcinoma.

摘要

目的

探讨热休克蛋白27（HSP27）、热休克蛋白60（HSP60）、热休克蛋白70（HSP70）和热休克蛋白90α（HSP90α）在食管鳞状细胞癌（ESCC）及手术切缘组织（TIM）中的表达及其意义。

方法

采用EnVision免疫组化法和蛋白质印迹法检测168例ESCC组织标本及42例TIM组织标本中HSP27、HSP60、HSP70和HSP90α的表达情况，比较其阳性染色率，并探讨它们的表达与ESCC临床病理特征之间的相关性。

结果

ESCC组织和TIM组织中HSP27、HSP60、HSP70和HSP90α的阳性染色率分别为62.0%和42.1%、92.7%和63.2%、57.9%和22.2%、33.7%和18.5%。ESCC组织与TIM组织中HSP60和HSP70的表达差异有统计学意义（P<0.01），而HSP27和HSP90α的表达差异无统计学意义（P>0.05）。HSP27的阳性染色率随ESCC分化程度降低而下降（P<0.05）。