利用GAL4增强子捕获技术靶向斑马鱼的神经回路。

Targeting neural circuitry in zebrafish using GAL4 enhancer trapping.

作者信息

Scott Ethan K, Mason Lindsay, Arrenberg Aristides B, Ziv Limor, Gosse Nathan J, Xiao Tong, Chi Neil C, Asakawa Kazuhide, Kawakami Koichi, Baier Herwig

机构信息

Department of Physiology, University of California, San Francisco, California 94158, USA.

出版信息

Nat Methods. 2007 Apr;4(4):323-6. doi: 10.1038/nmeth1033. Epub 2007 Mar 18.

DOI:10.1038/nmeth1033

PMID:17369834

Abstract

We present a pilot enhancer trap screen using GAL4 to drive expression of upstream activator sequence (UAS)-linked transgenes in expression patterns dictated by endogenous enhancers in zebrafish. The patterns presented include expression in small subsets of neurons throughout the larval brain, which in some cases persist into adult. Through targeted photoconversion of UAS-driven Kaede and variegated expression of UAS-driven GFP in single cells, we begin to characterize the cellular components of labeled circuits.

摘要

我们展示了一个先导增强子捕获筛选实验，该实验利用GAL4在斑马鱼内源性增强子所决定的表达模式中驱动与上游激活序列（UAS）相连的转基因的表达。所呈现的表达模式包括在整个幼体大脑中少量神经元亚群中的表达，在某些情况下这种表达会持续到成体阶段。通过对UAS驱动的Kaede进行靶向光转化以及在单细胞中对UAS驱动的GFP进行斑驳表达，我们开始对标记回路的细胞成分进行表征。